{"created":"2023-06-20T16:47:59.552025+00:00","id":19781,"links":{},"metadata":{"_buckets":{"deposit":"a3b93ed4-d536-461e-a132-930da16f4bdc"},"_deposit":{"created_by":3,"id":"19781","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19781"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019781","sets":["14:2667:4459"]},"author_link":["33645","33647","33644","33646","33643"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：細胞膜間物質輸送の分子機構を解明する為に、VSOPの系を全原子モデルでMD計算しVSOPのopen構造とclosed構造を構築し解析した。その結果、open構造ではS4へリックスに特有なARG残基が様々なコンフォメーションでS3及びS1のGLU残基と塩橋対をなしてプロトン移動パスを構成することを示唆する結果を得る事ができた。これに加えて、closed構造では実験で報告された塩橋ペアとは異なるARGとGLUの塩橋対で構造を安定化して全く水分子を透過させない構造を得る事ができた。VSOPの電位依存的ゲーティング分子機構についての検証研究をこの結果を基に進めてゆく予定である。\n研究成果の概要（英文）：Voltage Sensor Only Protein (VSOP) having simple and small structure would be a superior model system than the Kv-channel protein to elucidate a material transport molecular mechanism across bio-membrane. We studied the mechanism of VSOP using molecular dynamics simulation method. We then focused the voltage-dependent proton conduction mechanisn On the basis of the hypothesis where for activation, S4 helix vertically moves against membrane, we constructed a lots of VSOP structures with different S4 configUrations by molecular modeling and long-time molecular dynamics simulations. We then obtained several important structures. An open structure candidate of VSOP which shows proton conduction pass along with typical salt-bridges between Arginine and Glutamine, also a structure candidate of closed VSOP which indicates completely inhibition of water transporting. Those structures should be useful to investigate the intrinsic transporting mechanism of membrane proteins.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2015～2017;   課題番号：15K07037;   研究分野：生物物理;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K07037/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者/研究分担者/連携研究者/連携研究者/連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"YONEZAWA,Yasushige"},{"subitem_text_language":"en","subitem_text_value":"TAKANO,Yu"},{"subitem_text_language":"en","subitem_text_value":"NAKAGAWA,Atushi"},{"subitem_text_language":"en","subitem_text_value":"OKAMURA,Yasushi"},{"subitem_text_language":"en","subitem_text_value":"TAKESITA,Kouhei"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所; 教授"},{"subitem_text_value":"広島市立大学院医学情報研究科; 教授"},{"subitem_text_value":"大阪大学蛋白質研究所; 教授"},{"subitem_text_value":"大阪大学医学研究科; 教授"},{"subitem_text_value":"大阪大学蛋白質研究所; 特任助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"米澤, 康滋"},{"creatorName":"ヨネザワ, ヤスシゲ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"鷹野, 優"},{"creatorName":"タカノ, ユウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中川, 敦史"},{"creatorName":"ナカガワ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"岡村, 康司"},{"creatorName":"オカムラ, ヤスシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"竹下, 浩平"},{"creatorName":"タケシタ, コウヘイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-22"}],"displaytype":"detail","filename":"15K07037seika.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K07037seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19781/files/15K07037seika.pdf"},"version_id":"5e9286a1-4c34-47e9-b7d2-b0b0d440eb28"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"生体膜間物質輸送","subitem_subject_scheme":"Other"},{"subitem_subject":"分子動力学シミュレーション","subitem_subject_scheme":"Other"},{"subitem_subject":"プロトン透過","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"計算科学シミュレーションによる膜電位依存性チャネルVSOPの動作機構研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"計算科学シミュレーションによる膜電位依存性チャネルVSOPの動作機構研究"},{"subitem_title":"A Simulation Study on the Mechanism of Membrane Voltage Depended Protein VSOP","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-22"},"publish_date":"2018-11-22","publish_status":"0","recid":"19781","relation_version_is_last":true,"title":["計算科学シミュレーションによる膜電位依存性チャネルVSOPの動作機構研究"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:34:18.662756+00:00"}