{"created":"2023-06-20T16:47:59.416227+00:00","id":19778,"links":{},"metadata":{"_buckets":{"deposit":"3af4b298-6d7a-4105-92be-c5da5a49e252"},"_deposit":{"created_by":3,"id":"19778","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19778"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019778","sets":["14:2667:4459"]},"author_link":["33636","33638","33637","33639"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究では、ゼニゴケをモデルとする遺伝子機能解析を促進するため、CRISPR RNAおよびCas9タンパク質の複合体のマイクロインジェクションによるゲノム編集法の開発を目指した。標的とする遺伝子として、気室形成制御因子NOPPERAB01(NOP1)を選択した。NOP1が欠失すると、葉状体に気室が形成されず、葉状体表面の緑色が薄くなるとともに表面が滑らかになるので、変異表現型を容易に観察できる。NOP1遺伝子を標的とするCRISPR/Cas9複合体を導入したところ、nop1変異表現型を示す株を得た。この株の標的配列中に5塩基対の欠失が認められ、ゲノム編集を確認した。\n研究成果の概要（英文）：DNA-free genome editing allows target-specific manipulation of a given genome. CRISPR RNA and Cas9 protein can be directly introduced into target cells and lead to genome-editing. We have successfully performed DNA-free genome editing mediated by microinjection of CRISPR/Cas9 complex in the liverwort Marchantia polymorpha. NOPPERABO1 (NOP1), of which loss-of-function mutation causes impaired air-chamber formation and thus can be found readily, was selected as a target gene. Custom gRNA and commercially available Cas9 protein were first allowed to form RNA-protein complex and then injected into single-cell sporelings by a laser thermal microinjector. One of the thalli grown from microinjected sporelings formed a sector that showed the nopl mutant phenotype, and DNA isolated from the sector showed 5-bp deletion in the target coding sequence. This is the first demonstration of DNA-free genome-editing by microinjection in plants.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2015～2017;   課題番号：15K06902;   研究分野：plant molecular genetics;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K06902/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者/連携研究者/連携研究者/連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"YAMATO,Katsuyuki"},{"subitem_text_language":"en","subitem_text_value":"KOHCHI,Takayuki"},{"subitem_text_language":"en","subitem_text_value":"ISHIZAKI,Kimitsune"},{"subitem_text_language":"en","subitem_text_value":"TAKIKAWA,Yoshihiro"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"},{"subitem_text_value":"京都大学大学院生命科学研究科; 教授"},{"subitem_text_value":"神戸大学大学院理学研究科; 准教授"},{"subitem_text_value":"近畿大学先端技術総合研究所; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大和, 勝幸"},{"creatorName":"ヤマト, カツユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33636","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50293915","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"河内, 孝之"},{"creatorName":"コウチ, タカユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33637","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40202056","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"石崎, 公庸"},{"creatorName":"イシザキ, キミツネ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33638","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00452293","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"瀧川, 義浩"},{"creatorName":"タキカワ, ヨシヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33639","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60446010","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-22"}],"displaytype":"detail","filename":"15K06902seika.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K06902seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19778/files/15K06902seika.pdf"},"version_id":"831f0884-29ac-4485-835d-d818b3e8e2c0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RNA-protein complex","subitem_subject_scheme":"Other"},{"subitem_subject":"CRISPR/Cas9","subitem_subject_scheme":"Other"},{"subitem_subject":"microinjection","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"モデル生物ゼニゴケにおけるマイクロインジェクションを用いたゲノム編集技術の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"モデル生物ゼニゴケにおけるマイクロインジェクションを用いたゲノム編集技術の開発"},{"subitem_title":"Genome editing by microinjection in the model organism Marchantia polymorpha","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-22"},"publish_date":"2018-11-22","publish_status":"0","recid":"19778","relation_version_is_last":true,"title":["モデル生物ゼニゴケにおけるマイクロインジェクションを用いたゲノム編集技術の開発"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:34:21.570974+00:00"}