@techreport{oai:kindai.repo.nii.ac.jp:00019772, author = {芦田, 久}, month = {}, note = {研究成果の概要(和文):食品製造・加工過程において生成する2つの廃棄物を有効活用して、腸のビフィズス菌を増やす食品素材の生産を試みた。(1)食肉加工過程で生成するブタ胃ムチンの0-結合型糖鎖をビフィズス菌由来の酵素、あるいは菌体を用いて分解することにより、ビフィズス菌特異的な増殖活性のある2糖ガラクト-N-ビオースの生産を検討した。(2)梅干し製造過程で生成する梅酢由来の梅ポリフェノールによるビフィズス菌増殖効果を見出し、増殖の鍵酵素であるビフィズス菌由来の2種類のGH43エキソ-α-アラビノフラノシダーゼをクローニングした。梅ポリフェノールの腸内細菌フローラ改善効果について、マウスを用いて評価した。 研究成果の概要(英文):The production of prebiotics for intestinal bifidobacteria from two food wastes was studied. (1) By using bifidobacterial glycosidases or cells, bifidogenic disaccharide galacto-N-biose was released from wasted mucin, which contains abundant Q-glycans. A novel 6-sulfo-N acetylglucosaminidase, an essential enzyme for degradation of terminally sulfated glycans, was identified. A bifidobacterial strain defective in incorporation of galacto-N-biose was also screened, which is suitable for production of the disaccharide. (2) Mume-polyphenols ~epared from umesu, a waste of umeboshi production, was found to enhance bifidobacterial growth. The major phenolic compound, prunose, was assimilated by B. catenulatum and B. pseudocatenulat~ which express prunose-specific ferulate esterase. Arabinan-like polysaccharides was degraded by two novel GH43 exo-α-arabinofuranosidases from B. longum. Effects of orally administrated mume-polyphenols on intestinal microbiota was evaluated using mice., 研究種目:基盤研究(C); 研究期間:2015~2017; 課題番号:15K07448; 研究分野:微生物学; 科研費の分科・細目:, application/pdf}, title = {食品由来廃棄物を有効活用した新規ビフィズス菌増殖因子の創成}, year = {2018}, yomi = {アシダ, ヒサシ} }