{"created":"2023-06-20T16:47:58.303645+00:00","id":19759,"links":{},"metadata":{"_buckets":{"deposit":"fb1a543b-f3c3-4d29-9c61-da733b6f327f"},"_deposit":{"created_by":3,"id":"19759","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19759"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019759","sets":["14:2667:4459"]},"author_link":["33591"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：3種類の前立腺癌関連抗原（PTH-rP,EGFR,EZH2）HLA-A3拘束性ペプチドを1抗原3～5種類合成した。前立腺癌12例に対し各ペプチドにてCTLassayを施行したところEZH2ペプチドの1種のEZH2733-741に対し最もペプチド特異的CTLの誘導を確認した。次にCTLの誘導を認めたEZH2733-741にて刺激されたCD8+CTLsにて6時間51Cr release assayでHLA-A11,-A31,-A33陽性患者各２例にてHLA-A3拘束性ペプチド特異的cytotoxicityを確認した。\n研究成果の概要（英文）：3~5 prostate cancer-associated antigens (PTH-rP,EGFR,EZH2) -derived peptides that were prepared based on the binding motif to the HLA-A3 supertype alleles (HLA-A11, -A31, and -A33) were functionally screened for their potential to induce peptide-specific cytotoxic Tlymphocytes (CTLs) from peripheral blood mononuclear cells (PBMCs) of 12 HLA-A3 supertype allele+ prostate cancer patients. As a result, EZH2733-741 peptide was found to efficiently induce peptide-specific CTLs. To test cytotoxicity of CTLs by a standard 6-h 51Cr-release assay, CD8+ T cells were purified from EZH2733-741 peptide-stimulated PBMCs. The EZH2733-741 peptide-stimulated and purified CD8+ T cells from PBMCs of HLA-A3 supertype allele+ prostate cancer patients showed higher cytotoxicity against HLA-A3 supertype allele-expressing LNCaP prostate cancer cells than against parental LNCaP cells.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2015～2017;   課題番号：15K10612;   研究分野：泌尿器科学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K10612/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"MINAMI,Takafumi"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学泌尿器科; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"南, 高文"},{"creatorName":"ミナミ, タカフミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33591","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70340809","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"15K10612seika.pdf","filesize":[{"value":"194.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K10612seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19759/files/15K10612seika.pdf"},"version_id":"e1491877-09f5-4886-a084-1b4a0e48609e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ペプチドワクチン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"前立腺癌に対する癌ワクチン療法に応用しうるペプチドの同定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"前立腺癌に対する癌ワクチン療法に応用しうるペプチドの同定"},{"subitem_title":"Identification of peptides which can be applied to cancer vaccine therapy for patients with prostate cancer","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"19759","relation_version_is_last":true,"title":["前立腺癌に対する癌ワクチン療法に応用しうるペプチドの同定"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:34:48.429865+00:00"}