{"created":"2023-06-20T16:47:57.141431+00:00","id":19737,"links":{},"metadata":{"_buckets":{"deposit":"a7f7bb1a-beb4-4226-adbf-9ccac47dcc51"},"_deposit":{"created_by":3,"id":"19737","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19737"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019737","sets":["14:2667:4459"]},"author_link":["33535"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：EGFR遺伝子変異陽性肺癌患者の血漿34検体をdigital PCR法を用いてT790M耐性遺伝子変異、活性化遺伝子変異 (exon19とexon21)を測定した。13例はEGFR-TKIによる治療前後のペア検体であった。T790M耐性変異は事前検討した変異アレル頻度0.015%以上を陽性と定義した。すべての検体において活性化遺伝子変異が確認された。ペア検体13例中、治療開始前の検体で陽性は1例のみで、EGFR-TKI耐性後の検体では10例で陽性が確認され(76%)、非侵襲的な高感度アッセイによる高い検出率が得られた。\n研究成果の概要（英文）：We evaluated liquid biopsy assays by digital PCR for detection of T790M mutations of EGFR in EGFR mutation-positive NSCLC patients with acquired EGFR-TKI resistance.Plasma samples from 34 patients including 13 paired samples who developed progression during EGFR-TKI treatment were collected. Samples were deemed to be ddPCR-positive if more than 0.015% of mutant allele frequency in cell-free DNA. All plasma samples were genotyped successfully. For 13 paired samples, TKI-sensitizing and T790M mutations were detected in plasma of 13 (100%) and 10 (76%) patients, respectively. T790M mutation was detected in one patient with pretreatment plasma sample.Noninvasive genotyping ddPCR assay revealed that T790M was found in the majority of NSCLC patients who developed progression on EGFR-TKI.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2015～2017;   課題番号：15K21526;   研究分野：臨床腫瘍学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K21526/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KANEDA, Hiroyasu"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"金田, 裕靖"},{"creatorName":"カネダ, ヒロヤス","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33535","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50351599","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"15K21526seika.pdf","filesize":[{"value":"464.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K21526seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19737/files/15K21526seika.pdf"},"version_id":"c506e368-8d33-42d1-be02-7cd1dd5f03fa"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"バイオマーカー","subitem_subject_scheme":"Other"},{"subitem_subject":"分子標的治療","subitem_subject_scheme":"Other"},{"subitem_subject":"血漿DNA","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"血漿遊離DNAを用いたEGFR-TKI耐性機序の解明と臨床的有用性検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"血漿遊離DNAを用いたEGFR-TKI耐性機序の解明と臨床的有用性検討"},{"subitem_title":"Acquired resistance mechanism to EGFR-TKI using circulating cell-free tumor DNA","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"19737","relation_version_is_last":true,"title":["血漿遊離DNAを用いたEGFR-TKI耐性機序の解明と臨床的有用性検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:35:23.574696+00:00"}