{"created":"2023-06-20T16:47:57.077485+00:00","id":19736,"links":{},"metadata":{"_buckets":{"deposit":"fea98c3d-550a-4d4f-a444-570cfbb3ee2d"},"_deposit":{"created_by":3,"id":"19736","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19736"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019736","sets":["14:2667:4459"]},"author_link":["33534","33533"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：慢性胆汁うっ滞患者における胆汁酸代謝のメカニズムを解明するために、我々は胆道閉鎖症患者の摘出肝からマイクロダイセクション法を用いて組織肝細胞のmRNAを抽出し各分子のmRNAを測定した。その結果、胆汁酸合成律速酵素CYP7A1の発現が胆汁酸高値でありながら抑制されておらず、一方、FGFR4は活性化されていたが、ERKのリン酸化は抑制されていた。本研究は、慢性胆汁うっ滞患者においてFGF19による胆汁酸合成ネガティブフィードバック機構が破綻しており、そのメカニズムは肝細胞内においてERK経路が抑制されている為であることを報告した最初の研究である。\n研究成果の概要（英文）：Regulatory mechanisms of bile acid synthesis under chronic cholestasis remain unclear. We analyzed molecules involving bile acid metabolism using liver and serum samples from 8 biliary atresia (BA) children and 4 non-cholestatic disease controls. CYP7A1 mRNA expression was not inhibited in BA microdissected hepatocyte-entriched tissue despite high serum bile acid concentrations. The FGF19 protein was synthesized in BA hepatocytes, and its serum concentration was elevated. FGFR4 was phosphorylated in BA livers. However, ERK phosphorylation was significantly decreased. his is the first study to demonstrated that the FGF19 pathway did not suppress bile acid synthesis due to downregulation of ERK pathway in BA patients.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2014～2017;   課題番号：26461608;   研究分野：小児肝臓病学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26461608/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者/研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KONDOU, Hiroki"},{"subitem_text_language":"en","subitem_text_value":"BESSHO, Kazuhiko"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部奈良病院; 講師"},{"subitem_text_value":"大阪大学大学院医学系研究科; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"近藤, 宏樹"},{"creatorName":"コンドウ, ヒロキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"別所, 一彦"},{"creatorName":"ベッショ, カズヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"26461608seika.pdf","filesize":[{"value":"232.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26461608seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19736/files/26461608seika.pdf"},"version_id":"363657a5-a9af-4081-9ea1-c3e66aaf2167"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"胆汁うっ滞","subitem_subject_scheme":"Other"},{"subitem_subject":"CYP7A1","subitem_subject_scheme":"Other"},{"subitem_subject":"FGF19","subitem_subject_scheme":"Other"},{"subitem_subject":"胆汁酸合成","subitem_subject_scheme":"Other"},{"subitem_subject":"SPRY2","subitem_subject_scheme":"Other"},{"subitem_subject":"HNF4α","subitem_subject_scheme":"Other"},{"subitem_subject":"microdisection","subitem_subject_scheme":"Other"},{"subitem_subject":"胆道閉鎖症","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"小児胆汁うっ滞性疾患における胆汁酸／糖・脂質代謝制御機構破綻のメカニズムの検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"小児胆汁うっ滞性疾患における胆汁酸／糖・脂質代謝制御機構破綻のメカニズムの検討"},{"subitem_title":"Investigation of aberrant regulation of bile acid, glucose, and lipid metabolism under chronic cholestatic state in pediatric liver disease.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"19736","relation_version_is_last":true,"title":["小児胆汁うっ滞性疾患における胆汁酸／糖・脂質代謝制御機構破綻のメカニズムの検討"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:35:25.273075+00:00"}