{"created":"2023-06-20T16:47:54.396643+00:00","id":19683,"links":{},"metadata":{"_buckets":{"deposit":"9f0675a9-fe50-4d81-bb62-cee3b2fd950b"},"_deposit":{"created_by":3,"id":"19683","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19683"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019683","sets":["14:2667:4459"]},"author_link":["33418"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2017)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：神経細胞では外部からのシグナルに即座に応答するためにmRNAの転写後レベルでの制御が必須である。そして、シグナルに応答しmRNA翻訳のスイッチとなるのがRNA結合蛋白質である。本研究では、神経特異的RNA結合蛋白質HuDが標的mRNAと共に適切な場まで輸送されている間のmRNA翻訳OFF状態を維持する機構について当研究室独自の生化学的手法を用いて解析を行った。HuDとの相互作用が知られている既知因子（KIF3A、IMP1、SMN）についてin vitro翻訳系を用いてmRNAの翻訳に対する効果を評価した。その結果、SMNがHuDとの結合依存的にHuDの翻訳活性化能を阻害することを明らかにした。\n研究成果の概要（英文）：In neurons, the post-transcriptional regulation of gene expression is essential for the immediate response to external signals. Then, RNA binding protein is a switch for mRNA translation in response to signal. In this study, we analyzed molecular mechanism of maintaining the mRNA translation \"OFF\" state while the neuron specific RNA binding protein HuD is transported to the appropriate \"place\" together with the target mRNA ,using the unique biochemical technique of our laboratory. For the known factors (KIF3A, IMP1, SMN) whose interactions with HuD, the effect on translation of mRNA was evaluated using an in vitro translation system. As a result, it was revealed that SMN inhibits the translational activation ability of HuD depending on the binding ability of SMN with HuD.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2015～2017;   課題番号：15K18480;   研究分野：分子生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K18480/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FUKAO, Akira"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学薬学部; 助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"深尾, 亜喜良"},{"creatorName":"フカオ, アキラ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"33418","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50733979","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-20"}],"displaytype":"detail","filename":"15K18480seika.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"15K18480seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/19683/files/15K18480seika.pdf"},"version_id":"ec61220e-8af0-4367-8f0b-ae4659edf549"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"翻訳制御","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA結合蛋白質","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"神経特異的RNA結合蛋白質HuDが担う部位特異的翻訳制御機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"神経特異的RNA結合蛋白質HuDが担う部位特異的翻訳制御機構の解析"},{"subitem_title":"The regulation of local translation by neuronal RNA binding protein HuD","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4459"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-11-20"},"publish_date":"2018-11-20","publish_status":"0","recid":"19683","relation_version_is_last":true,"title":["神経特異的RNA結合蛋白質HuDが担う部位特異的翻訳制御機構の解析"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:36:11.165370+00:00"}