{"created":"2023-06-20T16:47:29.908597+00:00","id":19191,"links":{},"metadata":{"_buckets":{"deposit":"35b06bdb-d0c0-4911-a14f-99bdb7daa19c"},"_deposit":{"created_by":3,"id":"19191","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"19191"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00019191","sets":["14:923:1379:4406"]},"author_link":["42155","42156","42154","42153"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"〈原著〉軟腐朽を起こすGraphium sp. M-1-9のラッカーゼの諸性質について"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"51","bibliographicPageEnd":"50","bibliographicPageStart":"35","bibliographic_titles":[{"bibliographic_title":"近畿大学農学部紀要"},{"bibliographic_title":"MEMOIRS OF THE FACULTY OF AGRICULTURE OF KINDAI UNIVERSITY","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[Synopsis]An extracellular laccase was isolated, purified, and partially characterized from the culture medium of Grphium sp. M-1-9, which causes soft rot in Japanese beech wood. Laccase producted by this fungus in cultures supplemented with copper sulfate was purified 60-fold by ammonium-sulfate precipitation, Sephadex G-100 gel-filtration chromatography, DEAE Sepharose ion-exchange chromatography, and Superdex G-75 gel-filtration chromatograpgy, with a yield of 3.3%. Native and SDS-PAGE showed an active band with a molecular mass of approximately 67,000. Purified laccase maintained 70-80% stability at pH 6-10 for 180 min and at 30-40°C for 120 min. The optimum pH for the oxidation of 2,2´-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) was pH 3, while that for the oxidation of 2,6-dimethoxyphenol (DMP), guaiacol, and syringaldazine was pH 6. Kinetic constants (Km and kcat/km) showed that 2,6-DMP and syringaldazine were more effective substrates than ABTS and guaiacol. Enzyme activity was completely inhibited by sodium azide or L-cysteine at a concerntration of 1 mM. Laccase activity was also inhibited by 5 mM SDS.\\\n[要約]ブナ材に軟腐朽を起こすGraphiumsp. M-1-9を、硫酸銅を添加したブナ木片培地で培養し、ラッカーゼを分離・精製し、その諸性質を検討した。硫安沈殿、ゲルロ過クロマトグラフィー、イオン交換クロマトグラフィーにより60倍に精製されたラッカーゼの収量は3.3％であった。SDS-PAGEにより、分子量は約67000であった。精製したラッカーゼはpH 6-10では180分間、また30-40℃では120分間、70-80％の活性を維持した。最適pHは基質により異なっており、2,2ʹ-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)ではpH 3、2,6-dimethoxyphenol(DMP)、guaiacol,、syringaldazineではpH 6であった。Kmとkcat/Kmより2,6-DMP とsyringaldazineは、ABTS とguaiacolより効率的な基質であった。酵素活性は1 mM.のアジ化ナトリウムあるいはL-システイン、5 mM SDSで阻害された。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学農学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21896267","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"TANAKA,Hiromi"},{"subitem_text_language":"en","subitem_text_value":"YAMAMOTO,Takanori"},{"subitem_text_language":"en","subitem_text_value":"Honda,Katsuki"},{"subitem_text_language":"en","subitem_text_value":"ITAKURA,Shuji"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 名誉教授"},{"subitem_text_value":"近畿大学大学院農学研究科応用生命化学専攻"},{"subitem_text_value":"近畿大学大学院農学研究科応用生命化学専攻"},{"subitem_text_value":"近畿大学大学院農学研究科応用生命化学専攻"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 裕美"},{"creatorName":"タナカ, ヒロミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山本, 貴義"},{"creatorName":"ヤマモト, タカノリ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"本田, 克樹"},{"creatorName":"ホンダ, カツキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"板倉, 修司"},{"creatorName":"イタクラ, シュウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-03-08"}],"displaytype":"detail","filename":"AN00064044-20180331-0035.pdf","filesize":[{"value":"460.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00064044-20180331-0035.pdf","url":"https://kindai.repo.nii.ac.jp/record/19191/files/AN00064044-20180331-0035.pdf"},"version_id":"ffab82ec-371d-4978-98b5-3513aa567bea"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"soft-rot fungus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"laccase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Graphium sp.","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"wood degradation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Originals〉Purification and partial characterization of laccase from the soft-rot fungus Graphium sp. M-1-9a","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Originals〉Purification and partial characterization of laccase from the soft-rot fungus Graphium sp. M-1-9a","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4406"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-03-08"},"publish_date":"2018-03-08","publish_status":"0","recid":"19191","relation_version_is_last":true,"title":["〈Originals〉Purification and partial characterization of laccase from the soft-rot fungus Graphium sp. M-1-9a"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T19:56:56.133753+00:00"}