@techreport{oai:kindai.repo.nii.ac.jp:00018882,
 author = {佐伯, 和弘 and 加藤, 暢宏 and 橋本, 周},
 month = {},
 note = {研究成果の概要（和文）：同一のPDMS製マイクロウェル内でウシ卵子の体外成熟受精および胚培養を行うためマイクロウェルに微小流路を設置し、1個ずつ卵子を導入、微小流路を通じて培養液のウェル内での交換が可能かを検討したが、流路に卵子がはまり込むようになった。このため、代替法としてガス調節剤とウォーターバスを用いた簡易体外受精について検討したところ、1本の試験管内に卵子を導入し、培養液をピペットで交換するだけで胚盤胞を生産できるシステムの開発に成功した。また、胚発生観察（TLC)装置で個別の胚の発生を詳細に観察した。その結果、卵割のタイミングを本TLC装置で調べることで、ウシ体外受精胚の胚盤胞への発生を予測できた。
研究成果の概要（英文）：We produced PDMS micro-wells connected with narrow canals. Bovine oocytes were introduced into the wells and medium was changed through the canals, but oocytes were trapped into the canals. Therefore, we developed simple bovine IVP systems. Bovine oocytes were introduced into test tubes filled with maturation medium. The oocytes were matured then only medium was changed by small pipets with IVF medium. The oocytes were fertilized, then medium was then changed with development medium. The IVF oocytes were cultured for 168 hours.The blastocyst rates were the same with the control method. We also examine the cleavage timing of bovine IVF oocytes with small TLC device which we originally produced. We can predict blastocyst development by examining the cleavage timing., 研究種目：基盤研究(C);  研究期間：2014～2016;   課題番号：26450461;   研究分野：獣医繁殖学;   科研費の分科・細目：, application/pdf},
 title = {自動体外受精胚作製装置の開発},
 year = {2017},
 yomi = {サエキ, カズヒロ and カトウ, ノブヒロ and ハシモト, シュウ}
}