{"created":"2023-06-20T16:47:13.382928+00:00","id":18863,"links":{},"metadata":{"_buckets":{"deposit":"5ce5597d-f39b-4cd3-9858-35682dcf1926"},"_deposit":{"created_by":3,"id":"18863","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"18863"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018863","sets":["14:2667:4374"]},"author_link":["31934"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2016)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究では、ドナー細胞の性質・種類に問われないiPS細胞の誘導及び培養法の開発を試みた。実験には様々な生理現象に関わっており、また種保存性が高いβ-cateninおよび転写コアクティベータCBPに着目した。CBP/β-catenin経路の活性化によってマウスだけでなく、ヒト細胞においても多能性幹細胞へのリプログラムを促進することを明らかにした。また、マウス多能性幹細胞の未分化維持に対して、CBP/β-cateninの活性化は基底状態を強く正に制御することが観察された。以上のことから、CBP/β-catenin経路の活性化は、再現性が高いかつ効率的なiPS細胞の誘導及び培養法であると考えられる。\n研究成果の概要（英文）：Wnt/beta-catenin signaling pathway is conserved in numerous animal species, and greatly involved in homeostasis mechanism as well as various body developments. We hypothesized the involvement of Wnt/beta catenin, especially CBP/beta catenin signaling pathway is deeply associated with the reprograming of pluripotent stem cell（PSC）. In this study, we tried to develop the inducing and culture method of iPS cells independent of characteristics of donor cells. It was promoted reprogramming of fibroblasts to PSC not only in mice but also in human cells by activating the CBP/β-catenin pathway. In addition, it was observed that activation of CBP/β-catenin strongly and positively regulates the maintenance of undifferentiation of mouse PSC as naive state. These results suggest that activation of the CBP/β-catenin pathway is thought to be a highly efficient iPS cell induction and culture method.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2014～2016;   課題番号：26861216;   研究分野：幹細胞生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26861216/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"TAKEHARA, Toshiyuki"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部; 助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"竹原, 俊幸"},{"creatorName":"タケハラ, トシユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-01"}],"displaytype":"detail","filename":"26861216seika.pdf","filesize":[{"value":"356.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26861216seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18863/files/26861216seika.pdf"},"version_id":"be9f9b3d-b504-4ea1-bf5f-e8294fe7f8a6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"iPS細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"β-catenin","subitem_subject_scheme":"Other"},{"subitem_subject":"CBP/p300","subitem_subject_scheme":"Other"},{"subitem_subject":"リプログラム","subitem_subject_scheme":"Other"},{"subitem_subject":"多能性幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"転写コアクティベーター","subitem_subject_scheme":"Other"},{"subitem_subject":"ES細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"β-catenin/CBP経路の選択的な活性化による新規iPS細胞作製法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"β-catenin/CBP経路の選択的な活性化による新規iPS細胞作製法の開発"},{"subitem_title":"The activation of CBP/beta-catenin signal pathway promotes reprogramming of pluripotency on mice and human cells.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4374"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-01"},"publish_date":"2017-11-01","publish_status":"0","recid":"18863","relation_version_is_last":true,"title":["β-catenin/CBP経路の選択的な活性化による新規iPS細胞作製法の開発"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:46:41.998342+00:00"}