{"created":"2023-06-20T16:47:11.844669+00:00","id":18830,"links":{},"metadata":{"_buckets":{"deposit":"44635405-f6d1-45ac-a268-e39c708099e1"},"_deposit":{"created_by":3,"id":"18830","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"18830"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018830","sets":["14:2667:4374"]},"author_link":["31833"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2016)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：イオン液体は常温で液体の有機塩であり、既存の水系・有機系溶媒に換わって生体触媒の新たな反応溶媒として注目される。しかしイオン液体による細菌宿主の生育阻害や酵素の失活が問題であり、解決策が模索されている。本研究では、親水性イオン液体[BMIM][Cl]耐性Bacillus amyloliquefaciens CMW1を発見した。10% (631 mM)[BMIM][Cl]で多くの細菌は生育できないが、本菌株は良好に生育した。ゲノムDNAを解読して、細菌のイオン液体耐性に関与する遺伝子を推定した。本菌株のプロテアーゼは各種イオン液体に耐性を示し、塩耐性、アルカリ耐性、有機溶媒耐性をも示した。\n研究成果の概要（英文）：Ionic liquids are various kinds of molten organic salts, which are composed of a bulky asymmetric cation and a small anion. Extensive studies of biocatalytic reactions using ionic liquids have been carried out. An ionic liquid [BMIM][Cl] improved the digestibility of biomasses but was toxic to the bacteria. [BMIM][Cl] deactivated various enzymes. Therefore, there exists demand for a [BMIM][Cl]-tolerant bacterial host and an enzyme. In this study, Bacillus amyloliquefaciens CMW1 was isolated from a Japanese fermented soybean paste. Strain CMW1 grows in the presence of 10% (v/v, 631 mM) [BMIM][Cl], which inhibits the growth of various microorganisms. The genome sequence of strain CMW1 was determined and allowed for characterization of the molecular mechanism of the ionic liquid tolerance. A protease from strain CMW1 functioned in various hydrophilic and hydrophobic ionic liquids, and exhibited tolerance to ionic liquid and halo, alkaline, and organic solvents.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2014～2016;   課題番号：26870731;   研究分野：応用微生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26870731/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KURATA, Atsushi"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"倉田, 淳志"},{"creatorName":"クラタ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-01"}],"displaytype":"detail","filename":"26870731seika.pdf","filesize":[{"value":"258.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26870731seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18830/files/26870731seika.pdf"},"version_id":"e1d39db8-dcf1-46ae-8288-3ff7891eafcd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"イオン液体","subitem_subject_scheme":"Other"},{"subitem_subject":"Bacillus","subitem_subject_scheme":"Other"},{"subitem_subject":"属細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"イオン液体耐性細菌","subitem_subject_scheme":"Other"},{"subitem_subject":"浸透圧ストレス","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノム解析","subitem_subject_scheme":"Other"},{"subitem_subject":"次世代シーケンサー","subitem_subject_scheme":"Other"},{"subitem_subject":"イオン液体耐性酵素","subitem_subject_scheme":"Other"},{"subitem_subject":"プロテアーゼ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"イオン液体耐性細菌のゲノム解読と耐性機構の解明、有用酵素の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"イオン液体耐性細菌のゲノム解読と耐性機構の解明、有用酵素の開発"},{"subitem_title":"Genome sequence of an ionic liquid-tolerant bacteria and exploration of ionic liquid-tolerant enzyme","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4374"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-01"},"publish_date":"2017-11-01","publish_status":"0","recid":"18830","relation_version_is_last":true,"title":["イオン液体耐性細菌のゲノム解読と耐性機構の解明、有用酵素の開発"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:52:00.641233+00:00"}