{"created":"2023-06-20T16:47:11.522233+00:00","id":18823,"links":{},"metadata":{"_buckets":{"deposit":"921a21ac-7e5e-4b3a-83c9-a901ea118d67"},"_deposit":{"created_by":3,"id":"18823","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"18823"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018823","sets":["14:2667:4374"]},"author_link":["31818","31817","31816"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2016)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：本研究では、マウスとブタの胚盤胞を用いて、凍結乾燥保存を試みた。マウス胚盤胞を用いた検討では、トレハロース添加溶液を用い元に戻す時に多量の等張液を用いることで、凍結乾燥後の胚盤胞の状態が改善されることがわかった。ブタ胚盤胞ではトレハロース添加区を含めいずれの検討区においても、凍結乾燥後、元に戻しても胚盤胞の形態は回復しなかった。以上の結果から、マウス胚盤胞では凍結乾燥保存できる可能性が示され、ブタではより工夫が必要であることが示された。\n研究成果の概要（英文）：In this study, we examined whether pre-implantation stage embryos in mammals could be freeze-dried like sperm and somatic cells. When mouse blastocysts were freeze-dried, trehalose supplemented medium for freeze-drying and isotonic solution for rehydration were effective for their blastocoele recovery. For porcine blastocysts, no blastocysts recovered their blastocoele after rehydration in any approach.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2014～2016;   課題番号：26660261;   研究分野：動物発生工学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26660261/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者/連携研究者/研究協力者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"KATO, Yoko"},{"subitem_text_language":"en","subitem_text_value":"TANI,Tetsuya"},{"subitem_text_language":"en","subitem_text_value":"TAGUCHI,Misato"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 教授"},{"subitem_text_value":"近畿大学農学部; 講師"},{"subitem_text_value":"近畿大学大学院農学研究科; 博士前期課程修了（2016 年度）"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"},{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 容子"},{"creatorName":"カトウ, ヨウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"31816","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40278742","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"谷, 哲弥"},{"creatorName":"タニ, テツヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"31817","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70319763","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]},{"creatorNames":[{"creatorName":"田口, 美里"},{"creatorName":"タグチ, ミサト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"31818","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-11-01"}],"displaytype":"detail","filename":"26660261seika.pdf","filesize":[{"value":"460.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26660261seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18823/files/26660261seika.pdf"},"version_id":"2d8977ec-12b5-4380-a9d2-c0f3df83b571"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"凍結乾燥","subitem_subject_scheme":"Other"},{"subitem_subject":"初期胚","subitem_subject_scheme":"Other"},{"subitem_subject":"哺乳動物","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"哺乳動物初期胚の凍結乾燥保存の試み","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"哺乳動物初期胚の凍結乾燥保存の試み"},{"subitem_title":"Studies on the freeze-drying of mammalian preimplantataion embryos","subitem_title_language":"en"}]},"item_type_id":"8","owner":"3","path":["4374"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-11-01"},"publish_date":"2017-11-01","publish_status":"0","recid":"18823","relation_version_is_last":true,"title":["哺乳動物初期胚の凍結乾燥保存の試み"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:52:15.680309+00:00"}