{"created":"2023-06-20T16:46:57.001560+00:00","id":18554,"links":{},"metadata":{"_buckets":{"deposit":"ee8832b3-50f7-4daa-92f5-cd943b7c4bea"},"_deposit":{"created_by":3,"id":"18554","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"18554"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018554","sets":["14:923:2219:4354"]},"author_link":["31313"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"これまでの研究から、圧力の増加に伴い（i）力学的圧縮と（ii）熱力学的転移の２つのタイプの蛋白質構造変化が同時に進行することが分かっている。（i）力学的圧縮とは蛋白質が天然状態や中間体状態などの状態のまま圧縮される構造変化であるのに対し、（ii）熱力学的転移とは蛋白質が異なる状態へ転移する際の構造変化のことである。ここで、（ii）の過程によって現れる中間体が機能に重要であると指摘されており、この過程による構造変化を詳細に調べることが重要である。現在、高圧NMR という手法により、圧力依存的な構造変化の過程を、各アミノ酸残基の化学シフトの変化として調べることができる。従来はこの化学シフト変化を二次式解析法で解析し、上記の状態変化の情報を得ていた。一方筆者は、化学シフト－主成分解析（CS-PCA）という化学シフトデータの解析法を提案している。そこで今回、この方法を用いて圧力依存的な構造変化の解析を行い、従来法との結果の比較を行った。その結果、二次式解析法から得られた（ii）熱力学的転移の寄与の情報の中に（i）力学的圧縮の寄与が混入していたのに対し、CS-PCA法では（ii）の情報と（i）の情報が明確に分離されていることが分かった。\nAccording to the previous studies, the following two kinds of conformational changes of protein occur upon increase in pressure. One is（i） mechanical compression and the other is（ii）transition between thermodynamically distinct states. It is suggested that intermediate states accumulating in the pressureinduced conformational change via mechanism（ii） is relevant for its function. Thus, structural analysis of these states is important to understand the structure-function relationship. At present, pressure-dependent chemical shift changes of individual residues can be measured by using high-pressure NMR apparatus. Conventionally, such pressure-dependent chemical shift data were analyzed by fitting them to a quadratic equation to decompose the contributions from（i） and（ii）. On the other hand, I have suggested a distinct analytical method named as chemical shift-principal component analysis（CS-PCA）. In this paper, I applied a CS-PCA method to the pressure-dependent chemical shift data to decompose them into contributions from（i） and（ii） too, and compared the obtained results with those from the conventional quadratic analysis. It is found that the extracted information of the（ii） contribution by the conventional method was not completely clean because the（i） contribution was also involved therein. On the other hand, CS-PCA provided information of the（ii） contribution completely uncorrelated from that of the（i） contribution.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"SAKURAI,Kazumasa"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所高圧力蛋白質研究センター "}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"櫻井, 一正"},{"creatorName":"サクライ, カズマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-08-04"}],"displaytype":"detail","filename":"AA11574630-20170331-0001.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20170331-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/18554/files/AA11574630-20170331-0001.pdf"},"version_id":"7856eade-c94e-4edf-bc37-416fc385654c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"NMR","subitem_subject_scheme":"Other"},{"subitem_subject":"蛋白質","subitem_subject_scheme":"Other"},{"subitem_subject":"圧力変性","subitem_subject_scheme":"Other"},{"subitem_subject":"化学シフト","subitem_subject_scheme":"Other"},{"subitem_subject":"主成分解析","subitem_subject_scheme":"Other"},{"subitem_subject":"nuclear magnetic resonance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pressure-induced denaturation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chemical shift, principle component analysis.","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Original Papers〉圧力NMR化学シフトデータの二次式回帰分析法と主成分分析法の解析結果の比較","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Original Papers〉圧力NMR化学シフトデータの二次式回帰分析法と主成分分析法の解析結果の比較"},{"subitem_title":"Comparison between conventional, quadratic analysis and chemical-shift-PCA on data of pressure-dependent chemical shift changes","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["4354"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-08-04"},"publish_date":"2017-08-04","publish_status":"0","recid":"18554","relation_version_is_last":true,"title":["〈Original Papers〉圧力NMR化学シフトデータの二次式回帰分析法と主成分分析法の解析結果の比較"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2023-06-20T21:57:08.999369+00:00"}