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Protective effect of L-alanine on copper- and a fragment of amyloid β peptide-mediated neurotoxicity in PC 12 cells
https://kindai.repo.nii.ac.jp/records/18489
https://kindai.repo.nii.ac.jp/records/18489746c5ad4-0a7a-4d10-9e9f-766a2a0ba355
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AN10074306-20170228-0023.pdf (644.2 kB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||||||
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| 公開日 | 2017-06-20 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Protective effect of L-alanine on copper- and a fragment of amyloid β peptide-mediated neurotoxicity in PC 12 cells | |||||||||||||
| 言語 | en | |||||||||||||
| 著者 |
Minami, Takeshi
× Minami, Takeshi
× Sakamoto, Yamato
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| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| キーワード | ||||||||||||||
| 主題 | copper, amyloid β-peptide fragment, L-alanine, PC12 cell, cell viability, neurite | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||||
| 著者 所属 | ||||||||||||||
| 値 | 近畿大学 | |||||||||||||
| 著者 所属 | ||||||||||||||
| 値 | 近畿大学 | |||||||||||||
| 著者所属(翻訳) | ||||||||||||||
| 値 | Laboratory of Environmental Biology, Department of Life Science, School of Science & Engineering, Kindai University | |||||||||||||
| 著者所属(翻訳) | ||||||||||||||
| 値 | Laboratory of Environmental Biology, Department of Life Science, School of Science & Engineering, Kindai University | |||||||||||||
| 版 | ||||||||||||||
| 出版タイプ | NA | |||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||||||
| 出版者 名前 | ||||||||||||||
| 出版者 | 近畿大学理工学総合研究所 | |||||||||||||
| 書誌情報 |
理工学総合研究所研究報告 en : Annual reports by Research Institute for Science and Technology 号 29, p. 23-31, 発行日 2017-02-28 |
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| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 09162054 | |||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | Copper is one of the key metals in Alzheimer’s disease (AD) as it induces neurotoxicity. Chelation therapy to remove abnormal copper from the central nervous system has been attempted for AD. In the present study, the potential of L-alanine chelation therapy to remove copper toxicity was assessed. The cell viability of PC12 cells, a neuron-like cell, decreased by treatment with copper and amyloid β-peptide fragment (25-35) (AβF). When the protective effect of seventeen amino acids was tested, L-alanine, L-cysteine, Lglycine, and L-valine had protective effects, and L-alanine was the most effective in a dose-dependent manner. Furthermore, as the degree of polymerization of L-alanine increased, the protective effect decreased. In addition, D-alanine had no effect. It was effective even if L-alanine was added to the medium two hours before both copper and AβF addition, but was most effective when L-alanine was added one hour after both copper and AβF addition. The number of neurites per cell, the number of cells bearing neurites, and the length of neurites reduced by both copper and AβF treatment, and recovered by the addition of L-alanine. From the present results, it is suggested that L-alanine is a potential candidate for copper chelation therapy as it may bind to copper and reduce copper-induced neurotoxicity. | |||||||||||||
