{"created":"2023-06-20T16:46:39.131801+00:00","id":18165,"links":{},"metadata":{"_buckets":{"deposit":"fa2a24b1-c64d-4af9-8f49-c44e2b197f03"},"_deposit":{"created_by":29,"id":"18165","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18165"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018165","sets":["14:2667:4296"]},"author_link":["30602"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：完全養殖クロマグロ雄特徴的DNA多型を詳細に調査するため、F2世代雄の全血をより、ファージゲノムライブラリーを作成した。約6万のファージから雄特徴的配列を含む2つの独立のファージクローンを得た。2クローンの全塩基配列決定を行い、データベースを用いて他生物との相同解析を行った。その結果、2クローンは相同性が高いものの独立の配列である事、2クローン計34 kbpのゲノム配列にコーディングジーンがない事が明らかとなった。該当クローンには他生物で報告されているTRIM14遺伝子と相同性があった。該当領域を完全養殖F3雌雄で比較したところ、X染色体特徴的マーカー(97%雌陽性、n=32)を同定した。\n研究成果の概要（英文）：To determine the detail of male characteristic DNA marker, phage genomic DNA library was established using F2 generation of cultured Pacific Bluefin tuna male whole blood. Two phage clones containing male characteristic sequence were screened from 60,000 phages. Nucleotide sequence of the insert was identified and the resultant sequences were analyzed by international data base. It revealed that the two clones were highly homologous but they were independent sequence, and totally 34 kbp of the Pacific bluefin tuna genomic sequence did not contain coding gene. However, the sequence had similarity to TRIM14 gene. We determined male characteristic sequence region between F3 male and female, then found X chromosome characteristic marker (97% female positive, n=32).","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(Ｂ);  研究期間：2013～2015;   課題番号：25870937;   研究分野：分子生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25870937/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"AGAWA, Yasuo"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学水産研究所; 助教"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"阿川, 泰夫"},{"creatorName":"アガワ, ヤスオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-15"}],"displaytype":"detail","filename":"25870937seika.pdf","filesize":[{"value":"209.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"25870937seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18165/files/25870937seika.pdf"},"version_id":"37710880-8ed0-43e5-a250-66fe1719ba38"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"クロマグロ","subitem_subject_scheme":"Other"},{"subitem_subject":"雌雄判別DNAマーカー","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"クロマグロ産卵親群編成の為の、精度良い雌雄判別DNAマーカーの確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"クロマグロ産卵親群編成の為の、精度良い雌雄判別DNAマーカーの確立"},{"subitem_title":"Establishment of a high fidelity sex genotyping DNA marker of Pacific bluefin tuna for organization of spawning group.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-15"},"publish_date":"2016-10-15","publish_status":"0","recid":"18165","relation_version_is_last":true,"title":["クロマグロ産卵親群編成の為の、精度良い雌雄判別DNAマーカーの確立"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:07:18.924650+00:00"}