{"created":"2023-06-20T16:46:38.069026+00:00","id":18145,"links":{},"metadata":{"_buckets":{"deposit":"64891c9d-15a0-4a49-befe-9673196a20fa"},"_deposit":{"created_by":29,"id":"18145","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18145"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018145","sets":["14:2667:4296"]},"author_link":["30558"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：平成２６年度は、メチローマウス由来のES細胞や初期胚の解析を進めることで、細胞分化にともなって核内のDNAメチル化の強度やヘテロクロマチンパターンのパターンに大きな変化が生じることを定量的に証明し、Stem Cell Reports誌で発表することができた。２７年度は、それまでに確立した長時間高精細ライブセルイメージング技術を応用・改良し、単一ES細胞からコロニー形成に至るまで細胞が増殖してゆく過程、ES細胞からエピブラスト幹細胞が、エピブラスト幹細胞から始原生殖様細胞が誘導されてくる過程の核内メチル化DNAの変動を定量化するために、各種培養やイメージング条件の設定を行った。\n研究成果の概要（英文）：In 2014-2015, we elucidated that intensity and intra-nucleic pattern of methylated DNA were dynamically changed during cellular differentiation by using ES cells and preimplantation embryos. With these data, we have reported and published in Stem Cell Report Journal. In 2015-2016, by improving the above \"long-term and high-resolution live-cell imaging technology\", we determined the experimental conditions to capture and quantify the processes of colony formation of ES cells from single cell and of germ cell formation from ES cell.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2014～2015;   課題番号：26660250;   研究分野：発生工学、生殖生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26660250/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"YAMAGATA, Kazuo"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"山縣, 一夫"},{"creatorName":"ヤマガタ, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"30558","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"10361312","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-15"}],"displaytype":"detail","filename":"26660250seika.pdf","filesize":[{"value":"344.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26660250seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18145/files/26660250seika.pdf"},"version_id":"cfadbf4d-238c-4529-9f9e-71b6a294f644"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ＤＮＡメチル化","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞評価","subitem_subject_scheme":"Other"},{"subitem_subject":"メチロー","subitem_subject_scheme":"Other"},{"subitem_subject":"ライブセルイメージング","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"メチル化DNA可視化マウス（メチロー）を用いた細胞の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"メチル化DNA可視化マウス（メチロー）を用いた細胞の評価"},{"subitem_title":"Assesment of cellular quality using methylated DNA reporter mouse, MethylRO","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-15"},"publish_date":"2016-10-15","publish_status":"0","recid":"18145","relation_version_is_last":true,"title":["メチル化DNA可視化マウス（メチロー）を用いた細胞の評価"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:07:53.827163+00:00"}