{"created":"2023-06-20T16:46:37.937104+00:00","id":18143,"links":{},"metadata":{"_buckets":{"deposit":"a3982213-361b-4922-a797-222194d574a7"},"_deposit":{"created_by":29,"id":"18143","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18143"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018143","sets":["14:2667:4296"]},"author_link":["3854"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：従来法と比べゲノム編集技術の効果的なツールとして半数体ES細胞に着目し、マウス、ウサギ半数体胚およびES細胞の作出を試みた。本研究ではウサギにおいては半数体ES細胞の作出にまで至らなかったものの、マウス半数体ES細胞の作出が可能であった。ライブイメージング技術によって、半数体胚は低い発生能を有することを見出し、また半数体細胞特有の自発的な倍加現象はERK/MEKカスケードの阻害によって制御できることを示した。以上より、半数体ES細胞技術の開発が可能であるという結論が得られた。\n研究成果の概要（英文）：Haploid Embryonic stem cell(ESC) is more effective tool than conventional diploid ESC for genome editing. However, little is known about laboratory animal of haploid ESC besides the mouse. In this study, we aimed to obtain mouse and rabbit haploid ESC, and thus we tried to elucidate the characteristics of rabbit haploid embryo by live cell imaging technology. As a result, it showed to accumulation of developmental delays in each cell period of the produced rabbit haploid embryos are affecting the 8-cell stage or later development. On the other hand, we identified a new culture condition for inhibition of spontaneous polyploidy in haploid ESC. These findings indicated that haploid ESC has potential for new effective tool of genome editing in producing of human diseases animal model.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：挑戦的萌芽研究;  研究期間：2013～2015;   課題番号：25660259;   研究分野：繁殖・発生工学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25660259/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HOSOI, Yoshihiko"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"細井, 美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-15"}],"displaytype":"detail","filename":"25660259seika.pdf","filesize":[{"value":"762.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"25660259seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18143/files/25660259seika.pdf"},"version_id":"c7253c77-5180-46f4-9956-9f7dbdba68bb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"半数体細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ゲノム編集","subitem_subject_scheme":"Other"},{"subitem_subject":"胚性幹細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"ライブセルイメージング","subitem_subject_scheme":"Other"},{"subitem_subject":"ウサギ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ウサギ半数体胚性幹細胞の樹立と実用化への基礎研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ウサギ半数体胚性幹細胞の樹立と実用化への基礎研究"},{"subitem_title":"The application of rabbit haploid embryonic stem cell to genome editing technology","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-15"},"publish_date":"2016-10-15","publish_status":"0","recid":"18143","relation_version_is_last":true,"title":["ウサギ半数体胚性幹細胞の樹立と実用化への基礎研究"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:07:49.437461+00:00"}