{"created":"2023-06-20T16:46:35.384794+00:00","id":18102,"links":{},"metadata":{"_buckets":{"deposit":"c7954531-e956-4fb7-aa0e-c666eb361fce"},"_deposit":{"created_by":29,"id":"18102","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18102"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018102","sets":["14:2667:4296"]},"author_link":["30424"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：きのこの子実体形成には、各種プロテアーゼ群の活性化と不活化が深くかかわり、形態変化の制御因子として働いていることを報告してきた。シイタケゲノムデータベースよりプロテアーゼ様遺伝子群16種を抽出し、それぞれの遺伝子発現量を培養菌糸期と子実体原基形成期で相対定量比較し、子実体原基形成期では、g10056、g584、g1220、g4055遺伝子の発現量が顕著に増加し、子実体形成因子に関わることが示唆された。また、ブナシメジの子実体形成に深く関わるホスホラミドン感受性メタロプロテアーゼのアミノ酸配列を決定し、Fungalysin(Peptidase M38 family)であることを解明した。\n研究成果の概要（英文）：Fruit-body formation of Basidiomycetes was accelerated by the addition of carboxyl protease inhibitor (S-PI), and no occurrence of fruit-body by the addition of metallo protease inhibitors (Talopeptin, Phosphramidon, and EDTA).Several putative protease genes were selected using ForestGEN (L. edodes whole genome database). Among them, g876 (putative Eukaryotic aspartyl protease) showed high expression in mycelium stage of the fungus. In fruit-body (primordium)stage, g10056 (Eukaryotic aspartyl protease), g584, g1220 (Peptidase family 28) and g4055 (Peptidase family M13) were expressed higher than other putative protease genes. It seemed that g10056, g584, g1220 and g4055 have important role on fruit-body formation in L. edodes. On the other hand, phosphoramidon sensitive metalloprotease from Hypsizygus marmoreus was purified, and the gene was cloned. The protease gene sequence showed high similarity with Fungalysin (Peptidase M36 family).","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2014～2015;   課題番号：26870730;   研究分野：応用微生物学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26870730/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FUKUTA, Yasuhisa"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"福田, 泰久"},{"creatorName":"フクタ, ヤスヒサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"30424","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80609602","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-12"}],"displaytype":"detail","filename":"26870730seika.pdf","filesize":[{"value":"291.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26870730seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18102/files/26870730seika.pdf"},"version_id":"74755a14-c4cb-4d63-9da8-cc4400535f9f"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"プロテアーゼ","subitem_subject_scheme":"Other"},{"subitem_subject":"シイタケ","subitem_subject_scheme":"Other"},{"subitem_subject":"ブナシメジ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"シイタケとブナシメジ由来プロテアーゼの遺伝子解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シイタケとブナシメジ由来プロテアーゼの遺伝子解析"},{"subitem_title":"Gene analysis of proteases from Lentinula edodes and Hypsizygus marmoreus","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-12"},"publish_date":"2016-10-12","publish_status":"0","recid":"18102","relation_version_is_last":true,"title":["シイタケとブナシメジ由来プロテアーゼの遺伝子解析"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:09:04.435232+00:00"}