{"created":"2023-06-20T16:46:35.295743+00:00","id":18100,"links":{},"metadata":{"_buckets":{"deposit":"dae665f7-d9c5-4c01-ae1c-57618d15452a"},"_deposit":{"created_by":29,"id":"18100","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18100"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018100","sets":["14:2667:4296"]},"author_link":["4166"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：シロイヌナズナが発現するキチナーゼ様（CTL，Chitinase-like）タンパク質AtCTL1とAtCTL2およびイネ由来CTLタンパク質OsCTL1は，それぞれN末端シグナル配列，膜貫ドメイン，糖質加水分解酵素ファミリーのGH19キチナーゼ様ドメインとC末端領域から成る．本研究では，AtCTL1とAtCTL2のGH19キチナーゼ様ドメインのみをグルタチオンS－トランスフェラーゼおよびユビキチン様タンパク質との融合タンパク質として，大腸菌を用いた発現システムにより生産することに成功した．また，カラムクロマトグラフィーにより，SDS-PAGEにおいて均一なまでに精製した．\n研究成果の概要（英文）：AtCTL1, AtCTL2 and OsCTL1 are CTL, chitinase-like, proteins from Arabidopsis and rice (Oryza sativa). They consist of an N-terminal signal sequence, transmembrane region, GH19 chitinase-like domain, and C-teminal extension. We expressed the GH19 chitinase-like domains of AtCTl1 and AtCTL2 as fusion protein with glutathione S-transferase and SUMO protein, respectively, and purified them to homogeneity as judged by SDS/PAGE.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：若手研究(B);  研究期間：2014～2015;   課題番号：26850066;   研究分野：生化学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-26850066/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"OHNUMA, Takayuki"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学農学部; 准教授"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大沼, 貴之"},{"creatorName":"オオヌマ, タカユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-12"}],"displaytype":"detail","filename":"26850066seika.pdf","filesize":[{"value":"319.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"26850066seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18100/files/26850066seika.pdf"},"version_id":"af7fbc4d-4d60-44f7-805e-688122daab15"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"chitinase-like protein","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"植物の細胞壁構築に関与するキチナーゼ様タンパク質CTLの構造と機能","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"植物の細胞壁構築に関与するキチナーゼ様タンパク質CTLの構造と機能"},{"subitem_title":"Structure and function of chitinase-like proteins involved in cell wall biosynthesis.","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-12"},"publish_date":"2016-10-12","publish_status":"0","recid":"18100","relation_version_is_last":true,"title":["植物の細胞壁構築に関与するキチナーゼ様タンパク質CTLの構造と機能"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:09:00.860172+00:00"}