{"created":"2023-06-20T16:46:32.492553+00:00","id":18047,"links":{},"metadata":{"_buckets":{"deposit":"561d2c76-154d-4ae8-8714-e4939b04ed24"},"_deposit":{"created_by":29,"id":"18047","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18047"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018047","sets":["14:2667:4296"]},"author_link":["4143","30635","30634"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：翻訳開始段階を標的とし、翻訳を負に調節するmiRNAマシナリーの分子機構を生化学的手法で解析した。その結果、miRNAによる翻訳抑制機構がpoly(A)の短鎖化に依存しないことを2012年にPNAS誌上で発表した。次にmRNA pull-down法によりRNA-induced silencing complex(RISC)が形成されたmRNA上の翻訳因子の変化を詳細に調べた。その結果、miRNA機構がeIF4Aを標的として、翻訳開始を制御することを発見し、さらにHuDがmiRNAによる翻訳抑制を阻害することもつきとめ2014年にMolecular Cell誌上で発表した\n研究成果の概要（英文）：We showed that miRNA inhibits translation of the target mRNA in a deadenylation and PABP-independent manner at early time points (PNAS 2012)We next assessed the effect of miRISC on synergistic recruitment of translation initiation factors to target mRNAs by using direct biochemical assays. We show that miRISC promotes the release of both eIF4AI and eIF4AII but not eIF4E and eIF4G from target mRNAs and demonstrate that this is independent of deadenylation. Strikingly, both, the miRISC-induced release of eIF4A I and II from the cap binding complex (CBC) and the miRISC-induced inhibition of cap-dependent translation can be counteracted by the RNA binding protein HuD via a direct interaction of HuD with eIF4A. In summary, we propose that eIF4A I and II are key targets in the translation initiation control mechanisms by miRISC. These results were reported in Molecular Cell (2014).","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(B);  研究期間：2013～2015;   課題番号：25291007;   研究分野：RNA生化学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25291007/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"},{"subitem_text_value":"連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"FUJIWARA, Toshinobu"},{"subitem_text_language":"en","subitem_text_value":"TOMARI, Yukihide"},{"subitem_text_language":"en","subitem_text_value":"TAKIZAWA, Naoki"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学薬学部; 教授"},{"subitem_text_value":"東京大学分子細胞生物研究所; 教授"},{"subitem_text_value":"（公財）微生物化学研究会微生物化学研究所; 研究員"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤原, 俊伸"},{"creatorName":"フジワラ, トシノブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"泊, 幸秀"},{"creatorName":"トマリ, ユキヒデ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"滝沢, 直己"},{"creatorName":"タキザワ, ナオキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-17"}],"displaytype":"detail","filename":"25291007seika.pdf","filesize":[{"value":"725.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"25291007seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18047/files/25291007seika.pdf"},"version_id":"86d5b6c3-3b7a-4fe6-ab68-360657a4604d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"翻訳制御","subitem_subject_scheme":"Other"},{"subitem_subject":"microRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"RNA結合タンパク質","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"RNA結合タンパク質がファインチューニングする翻訳開始制御機構","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"RNA結合タンパク質がファインチューニングする翻訳開始制御機構"},{"subitem_title":"Fine-tuning of translation regulation system by RNA-binding proteins","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-17"},"publish_date":"2016-10-17","publish_status":"0","recid":"18047","relation_version_is_last":true,"title":["RNA結合タンパク質がファインチューニングする翻訳開始制御機構"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:10:38.459863+00:00"}