{"created":"2023-06-20T16:46:31.905559+00:00","id":18037,"links":{},"metadata":{"_buckets":{"deposit":"02d1d1a5-ab65-4288-be99-3b7462444227"},"_deposit":{"created_by":29,"id":"18037","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"18037"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00018037","sets":["14:2667:4296"]},"author_link":["3607","30609","3835"],"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2015)"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文）：我々は、分子進化において、ペプチドホルモンの生理活性構造が如何に保たれ、高い生理活性を獲得したのかを前駆体の立体構造形成との関連から開明することを目的とし、プロウログアニリンあるいはその他の種々の前駆体について、立体構造形成反応を調査した。その結果、i)成熟ペプチドホルモンの生理活性は前駆体の立体構造形成反応レベルで品質管理が行われていること、ii)分子進化を促進する立体構造部位が存在すること、iii)分子進化を抑制する立体構造部位が存在すること、iv)天然型の立体構造と異なる反応中間体を経由することにより、より高度な品質管理機構を発展させたことを明らかにした。\n研究成果の概要（英文）：To investigate how peptide hormones maintain/develop their biologically active conformation during molecular evolution, the folding of precursor proteins including prouroguanylin were estimated in vitro. Our results indicated that i)the stability of the biologically active conformation of the mature region is strongly related to the correct folding of their precursor proteins, ii)precursor protein possesses a region to accelerate molecular evolution, iii)precursor protein possesses a region to inhibit molecular evolution, iv)correct folding of prevursor protein requires mis-folded molecular species to establish the correct conformation of prouroguanylin","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究(C);  研究期間：2012～2015;   課題番号：24510310;   研究分野：蛋白質・ペプチド科学;   科研費の分科・細目：","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24510310/"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"連携研究者"},{"subitem_text_value":"連携研究者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HIDAKA, Yuji"},{"subitem_text_language":"en","subitem_text_value":"YAMAGUCHI, Hiroshi"},{"subitem_text_language":"en","subitem_text_value":"SHIMAMOTO Shigeru"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学理工学部; 教授"},{"subitem_text_value":"関西学院大学理工学部; 教授"},{"subitem_text_value":"近畿大学理工学部; 講師"}]},"item_8_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kindai University"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日高, 雄二"},{"creatorName":"ヒダカ, ユウジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"山口, 宏"},{"creatorName":"ヤマグチ, ヒロシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"島本, 茂"},{"creatorName":"シマモト, シゲル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-17"}],"displaytype":"detail","filename":"24510310seika.pdf","filesize":[{"value":"125.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"24510310seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/18037/files/24510310seika.pdf"},"version_id":"c89d4b3c-a694-496f-9187-b35ae1888bf8"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"シャペロン","subitem_subject_scheme":"Other"},{"subitem_subject":"ジスルフィド結合","subitem_subject_scheme":"Other"},{"subitem_subject":"フォールディング","subitem_subject_scheme":"Other"},{"subitem_subject":"ペプチドホルモン","subitem_subject_scheme":"Other"},{"subitem_subject":"前駆体","subitem_subject_scheme":"Other"},{"subitem_subject":"ウログアニリン","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ペプチドホルモン前駆体蛋白質を標的とした分子進化と生理活性成熟化機構の解明","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ペプチドホルモン前駆体蛋白質を標的とした分子進化と生理活性成熟化機構の解明"},{"subitem_title":"Relationship between precursor folding and molecular evolution of a peptide hormone","subitem_title_language":"en"}]},"item_type_id":"8","owner":"29","path":["4296"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-17"},"publish_date":"2016-10-17","publish_status":"0","recid":"18037","relation_version_is_last":true,"title":["ペプチドホルモン前駆体蛋白質を標的とした分子進化と生理活性成熟化機構の解明"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-06-20T22:10:19.437412+00:00"}