{"created":"2023-06-20T16:46:25.780688+00:00","id":17910,"links":{},"metadata":{"_buckets":{"deposit":"287d12bd-dabc-4ece-bb4d-24f72322d75d"},"_deposit":{"created_by":29,"id":"17910","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"17910"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00017910","sets":["14:923:1379:4295"]},"author_link":["42914","42912","42913","42911"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"49","bibliographicPageEnd":"36","bibliographicPageStart":"27","bibliographic_titles":[{"bibliographic_title":"近畿大学農学部紀要"},{"bibliographic_title":"MEMOIRS OF THE FACULTY OF AGRICULTURE OF KINKI UNIVERSITY","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"〈Synopsis〉Substrate-binding cleft of a family GH19 chitinase from rye seeds (RSC-c) was investigated by NMR spectroscopy, and the amino acid residue involved in the sugar residue binding was identified by site-directed mutagenesis. Mutation of Trp72 of RSC-c significantly reduced the binding affinity of the substrate, suggesting that the tryptophan residue is important for sugar residue binding not only at the mutation site but also in more extended region of the binding cleft.       ","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学農学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21896267","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"OKAZAKI, Yoko"},{"subitem_text_language":"en","subitem_text_value":"SHINYA, Shoko"},{"subitem_text_language":"en","subitem_text_value":"OHNUMA, Takayuki"},{"subitem_text_language":"en","subitem_text_value":"FUKAMIZO, Tamo "}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院農学研究科バイオサイエンス専攻バイオ分子化学研究室"},{"subitem_text_value":"近畿大学大学院農学研究科バイオサイエンス専攻バイオ分子化学研究室"},{"subitem_text_value":"近畿大学大学院農学研究科バイオサイエンス専攻バイオ分子化学研究室"},{"subitem_text_value":"近畿大学大学院農学研究科バイオサイエンス専攻バイオ分子化学研究室"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岡崎, 蓉子"},{"creatorName":"オカザキ, ヨウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"42911","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"新家, 粧子"},{"creatorName":"シンヤ, ショウコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"42912","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"大沼, 貴之"},{"creatorName":"オオヌマ, タカユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"42913","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"深溝, 慶"},{"creatorName":"フカミゾ, タモウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"42914","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-10-13"}],"displaytype":"detail","filename":"AN0006404-20160331-0027.pdf","filesize":[{"value":"2.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN0006404-20160331-0027.pdf","url":"https://kindai.repo.nii.ac.jp/record/17910/files/AN0006404-20160331-0027.pdf"},"version_id":"e8beae40-d9b2-448f-9f72-7b47f00bc653"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Family GH19 chitinase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chitin oligosaccharide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interaction","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈原著〉タンパク質NMR法を用いたライムギ種子Family GH19キチナーゼの基質結合部位に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈原著〉タンパク質NMR法を用いたライムギ種子Family GH19キチナーゼの基質結合部位に関する研究"},{"subitem_title":"〈Original 〉NMR study on the substrate-binding site of a family GH19 chitinase from rye seeds","subitem_title_language":"en"}]},"item_type_id":"2","owner":"29","path":["4295"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-10-13"},"publish_date":"2016-10-13","publish_status":"0","recid":"17910","relation_version_is_last":true,"title":["〈原著〉タンパク質NMR法を用いたライムギ種子Family GH19キチナーゼの基質結合部位に関する研究"],"weko_creator_id":"29","weko_shared_id":29},"updated":"2023-06-20T19:56:25.218200+00:00"}