{"created":"2023-06-20T16:46:13.604384+00:00","id":17669,"links":{},"metadata":{"_buckets":{"deposit":"70d2376a-364a-432e-9516-e6403ba9dc72"},"_deposit":{"created_by":29,"id":"17669","owners":[29],"pid":{"revision_id":0,"type":"depid","value":"17669"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00017669","sets":["14:923:1809:4275"]},"author_link":["29909","29910","29911","29912","29913","29914","29915","29916"],"control_number":"17669","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-07-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"24","bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kindai University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"For clinical applications of small interfering RNA (siRNA), chemical structure of siRNA should be optimized to be taken up into cells effectively, resistant to nuclease digestion, incorporated into RNA induced silencing complex (RISC) rapidly and correctly. They should also have minimized off-target effect and maximized turn over number. In this study, we investigated RNA interference (RNAi) efficiencies of siRNAs bearing 5’-amino-5’-deoxythymidine (T*) at 5’-end. The results showed that T* at the 5’-end of the sense strand did not interfere the processes of RISC loading complex (RLC) formation, loading to RISC or cleavage of the passenger strand by human argonaute2 (hAgo2) at all. On the other hand, T* at the 5’-end of the antisense strand gave a fatal damage to siRNA to show almost no silencing effect probably because RISC was destabilized by an electrostatic repulsion between the cationic charge of the ammonium group of T* and the cationic residues in MID domain pocket of hAgo2. These results strongly suggest that a modification of 5’-end of a sense strand with T* will suppress an off-target effect.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000017669","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科"},{"subitem_text_value":"近畿大学大学院産業理工学研究科産業理工学専攻"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 准教授"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"新貝, 恭広","creatorNameLang":"ja"},{"creatorName":"シンカイ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Shinkai, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"柏原, 慎一","creatorNameLang":"ja"},{"creatorName":"カシハラ, シンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Kashihara, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 啓史","creatorNameLang":"ja"},{"creatorName":"フジイ, ヒロフミ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Hirohumi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"峰松, 剛","creatorNameLang":"ja"},{"creatorName":"ミネマツ, ゴウ","creatorNameLang":"ja-Kana"},{"creatorName":"Minematsu, Go","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"吉永, 尚平","creatorNameLang":"ja"},{"creatorName":"ヨシナガ, ショウヘイ","creatorNameLang":"ja-Kana"},{"creatorName":"Yoshinaga, Shouhei","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"苗村, 円佳","creatorNameLang":"ja"},{"creatorName":"ナエムラ, マドカ","creatorNameLang":"ja-Kana"},{"creatorName":"Naemura, Madoka","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"神武, 洋二郎","creatorNameLang":"ja"},{"creatorName":"コウタケ, ヨウジロウ","creatorNameLang":"ja-Kana"},{"creatorName":"Kotake, Yojiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kindai University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-08-04"}],"displaytype":"detail","filename":"AA12013209-20160715-0001.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12013209-20160715-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/17669/files/AA12013209-20160715-0001.pdf"},"version_id":"49b6d792-4b3d-4147-8272-2081e3550904"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"chemical modification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene silencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"hAgo2","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RISC","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文・報告〉化学修飾small interfering RNA (siRNA)による遺伝子サイレンシング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文・報告〉化学修飾small interfering RNA (siRNA)による遺伝子サイレンシング","subitem_title_language":"ja"},{"subitem_title":"Gene Silencing by Chemically Modified Small Interfering RNA(siRNA)","subitem_title_language":"en"}]},"item_type_id":"2","owner":"29","path":["4275"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2016-08-03"},"publish_date":"2016-08-03","publish_status":"0","recid":"17669","relation_version_is_last":true,"title":["〈論文・報告〉化学修飾small interfering RNA (siRNA)による遺伝子サイレンシング"],"weko_creator_id":"29","weko_shared_id":-1},"updated":"2023-09-15T00:21:01.534250+00:00"}