| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2016-07-25 |
| タイトル |
|
|
タイトル |
<Review> Peripheral nerve storage and allotransplantation using green tea polyphenol in rats—its possibility for clinical application—Review of our works. |
|
言語 |
en |
| 著者 |
Kakinoki, Ryosuke
Ikeguchi, Ryosuke
Matsumoto, Taiichi
Nakayama, Ken
Fujita, Satoshi
Ohta, Soichi
Noguchi, Takashi
Kaizawa, Yukitoshi
Oda, Hiroki
Yurie, Hirofumi
Duncan, Scott FM
Hyon, Suong-Hyu
Tomiyama, Takashi
Hashimoto, Kazuhiko
Tanaka, Hiroki
Nishimura, Shunji
Akagi, Masao
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
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主題 |
peripheral nerve storage, polyphenol solution, sex-determining region of Ychromosome |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kurashiki Central Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Shizuoka Prefectural General Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kobe Central Municipal Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Graduate School of Medicine, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Boston University, School of Medicine |
| 著者 所属 |
|
|
値 |
Institute for Frontier Medical Science, Kyoto University |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 著者 所属 |
|
|
値 |
Department of Orthopedic Surgery, Kindai University Hospital |
| 版 |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 名前 |
|
|
出版者 |
Kindai University Medical Association |
| 書誌情報 |
en : ACTA MEDICA KINDAI UNIVERSITY
巻 41,
p. 1-8,
発行日 2016-06
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866092 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
[Abstract] Background. We examined whether peripheral nerve segments immersed in polyphenol solution could be preserved and allotransplanted into rats without using immunosuppressants. Methods. Sciatic nerve segments, 20 mm long, were harvested from male Lewis or DA rats and transplanted into 15-mm sciatic nerve deficits in Lewis rats immediately, or after polyphenol treatment (immersion in 1 mg/mL polyphenol solution for one week and in Dulbecco's modified Eagle's medium for three weeks more), without using immunosuppressants. In the FA group, DA rat nerve segments were transplanted into Lewis rats immediately. In the PA group, DA rat nerve segments were transplanted into Lewis rats after polyphenol treatment. In the FI group, Lewis rat nerve segments were transplanted immediately into Lewis rats. In the PI group, Lewis rat nerve segments were transplanted into Lewis rats after polyphenol treatment. Nerve regeneration was assessed electrophysiologically and histomorphologically. Nerve segments from male Lewis or DA rats were also transplanted into female Lewis rats as in the PI, PA, FI, and FA groups. Genomic DNA was extracted from each transplanted nerve segment and subjected to polymerase chain reaction amplification specific for the sex-determining region of Y-chromosome and β-actin genes. Immunostaining for S-100 and glial fibrillary acidic protein, and Y-chromosome-specific in situ hybridization were also performed. Results. Nerve regeneration in the PI and PA groups was similar to that in the FI group and was significantly greater than that in the FA group. The ratios of the Sry/β-actin polymerase chain reaction products for PA, PI, FI, and FA groups were 0.144, 0.294, 0.615, and 0, respectively. Some of the S-100-positive and glial fibrillary acidic protein-positive cells displayed Sry signals in the PI and FI groups, but not in the FA group. Conclusion. Some Schwann cells survived for one month in polyphenol-treated nerve segments that were transplanted allogeneically without immunosuppressants. |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
AA0050842X-20160600-0001.pdf (4.5 MB)
