{"created":"2023-06-20T16:43:55.788679+00:00","id":15016,"links":{},"metadata":{"_buckets":{"deposit":"8549e17b-bd63-4cca-b108-153af0c0c40f"},"_deposit":{"created_by":3,"id":"15016","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"15016"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00015016","sets":["14:923:1809:3957"]},"author_link":["27227"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-05-26","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"6","bibliographicPageStart":"1","bibliographicVolumeNumber":"5","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Pl9胚性癌腫細胞(PI9)は、マウス精巣に生じた奇形癌腫を株化したもので、幹細胞としての性質を持つ細胞株である。本研究では、コンビナトリアル・バイオエンジニアリングの手法の一つであるファージディスプレイ法により、未分化状態のP19を認識して結合するペプチドのスクリーニングを行い、幹細胞マーカーとなる分子の探索を行った。未分化状態のPl9をレチノイン酸を加えた培養液中で4日間浮遊培養し、神経細胞への分化誘導を行った。そして、直鎖12残基のペプチドを提示するファージペプチドライブラリーを神経細胞と混合し、これに結合しないファージを回収して未分化状態のPl9と結合し、これに結合するペプチドを単離した。スクリーニングによって得られたファージのPl9への結合の確認は、蛍光修飾された抗ファージ抗体を用いた蛍光イムノアッセイにより行った。スクリーニング後、ファージの提示するペプチドの配列には収束の傾向が見られた。このファージを単離して蛍光イムノアッセイを行ったところ、未分化状態のPl9に選択的に結合していることが示唆された。P 19 cell is a pluripotent stem cell of murine teratocarcinoma. When treated with retinoic acid, P 19 cells can be differentiated along a neural cell lineage in culture. To isolate peptides that bind to the stem cell, we employed a phage display technology with undifferentiated P 19 cells as the target. In order to r-educe non-specific binding of phages to the cell surface, the phage libraries were pre- adsorbed to the differentiated P 19 cells before each selection on the undifferentiated P 19 cells. After eight rounds of the selection、 No.28 phage displaying AしPST SSQMPQL-peptide was isolated. lmmunofluorescence analysis revealed that No.28 phage selectively binds to the undifferentiated P 19 cells, but not to the differentiated P 19 cells or SHSY cell line. The chemically synthesized peptide ALPST SSQMPQL presented on the No.28 phage efficiently inhibited the binding of No.28 phage to the undifferentiated P 19 cells. This result confirmed that No.28 phage binding to the cell was mediated by the displayed peptide. The identified peptide may be targeted to a marker expressed on the stem cell, and thus become a practical tool for the isolation of somatic stem cells.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部 "}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Morita, Yasutaka"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"metadata only access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_14cb"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森田,  資隆"},{"creatorName":"モリタ, ヤスタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Peptide library","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Phage display","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P19 embryonic carcinoma","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Stem cell marker","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ファージディスプレイ法によるP19胚性癌腫細胞認識","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ファージディスプレイ法によるP19胚性癌腫細胞認識"},{"subitem_title":"Screening and Characterizarion of P19 embryonic carcinoma stem cells binding peptide from phage display peptide library","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["3957"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-06-01"},"publish_date":"2016-06-01","publish_status":"0","recid":"15016","relation_version_is_last":true,"title":["ファージディスプレイ法によるP19胚性癌腫細胞認識"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T22:51:32.079828+00:00"}