@techreport{oai:kindai.repo.nii.ac.jp:00013985,
 author = {岸上,  哲士},
 month = {Jan},
 note = {研究成果の概要（和文): 本研究課題は, αチューブリンを含む非ヒストンタンパク質のアセチル化について初期発生における役割や制御機構を明らかにすることを目指した. 本研究では, まず卵子老化に伴うチューブリンのアセチル化レベルの経時変化を明らかにするとともに, ヒストン脱アセチル化酵素（HDAC）阻害剤を用いて卵子老化において特にクラスIIIのHDACの重要性を示唆した. また卵子の活性化に伴うヒストンおよび非ヒストンタンパク質の高アセチル化への誘導現象を明らかにした. このように本研究を通じて, 特に1細胞期におけるアセチル化制御の重要性について明らかにした.           研究成果の概要（英文): Histone Deacetylases (HDACs) are known to play important roles during preimplantation development. However, the roles of acetylation of non-histone proteins mouse embryos still remains poorly understood. In this study, we have focused on acetylation of alpha-tubulin and HDACs in terms of oocyte quality during one-cell stage. First, we found that treatment with NAM, nicotinamide, an inhibitor for Class III HDACs inhibited cellular fragmentation and spindle elongation after postovulatory in vitro aging. Also, the alpha-tubulin increased acetylation during aging, suggesting not only histone but non-histone protein acetylation also increases with oocyte aging. Further, once an oocyte has been activated, histone and nonhistone proteins are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation. Thus, we provide the evidence that protein acetylation play important roles for oocyte quality which affect subsequent embryonic development., 研究種目：基盤研究(C);  研究期間：2011～2013;   課題番号：23580416;   研究分野：農学;   科研費の分科・細目：畜産学・獣医学・基礎獣医学・基礎畜産学, application/pdf},
 title = {マウス初期発生におけるチューブリンのアセチル化制御機構と機能解析},
 year = {2013},
 yomi = {キシガミ, サトシ}
}