{"created":"2023-06-20T16:43:10.550067+00:00","id":13984,"links":{},"metadata":{"_buckets":{"deposit":"adeddaca-1738-4722-94bd-2a70b5a06832"},"_deposit":{"created_by":3,"id":"13984","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13984"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00013984","sets":["14:2667:2671:2672"]},"author_link":["25652","25653"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"In vitro growth of porcine growing oocytes"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2013. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文): ブタ発育途上卵母細胞の体外培養によるミトコンドリア活性の低下を防ぐため, アポトーシス抑制、ミトコンドリアへの長鎖脂肪酸の取込促進, ベータ酸化によるATP合成の促進効果を有するカルニチンによるブタ発育途上卵母細胞の発育能の改善に取り組んだ. L-カルニチンを添加することにより, ブタ発育途上卵母細胞の生存性が向上し, 超微小レベルでの形態観察により, 活性型ミトコンドリアの存在を確認した. さらに, 顆粒膜細胞に取り込まれたカルセインが卵母細胞に移行することを確認した. ブタ体外受精で多発する多精子受精を回避するため, 体外培養により発育した卵母細胞への顕微授精に成功した.           研究成果の概要（英文): We have focused attention on the mitochondrial activity of in vitro grown (IVG) oocytes and shown that L-Carnitine, which acts as a carrier of fatty acids across the inner mitochondrial membrane for subsequent beta-oxidation, supported the viability of growing oocytes. Moreover, we have successfully obtained fertilized ova by ICSI which developed from growing oocytes in vitro.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目：基盤研究（C）;  研究期間：2011～2013;   課題番号：23580397;   研究分野：農学;   科研費の分科・細目：畜産学・獣医学応用動物科学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://kaken.nii.ac.jp/d/r/30570949.ja.html"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"http://kaken.nii.ac.jp/d/r/70192739.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"},{"subitem_text_value":"研究分担者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"HASHIMOTO, Shu"},{"subitem_text_language":"en","subitem_text_value":"HOSOI, Yoshihiko"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 研究員"},{"subitem_text_value":"近畿大学生物理工学部; 教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"橋本,  周"},{"creatorName":"ハシモト, シュウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-03-31"}],"displaytype":"detail","filename":"KAKEN_23580397seika.pdf","filesize":[{"value":"202.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_23580397seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/13984/files/KAKEN_23580397seika.pdf"},"version_id":"4577257c-8d61-4964-ab8e-7e7d20227046"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"発生工学","subitem_subject_scheme":"Other"},{"subitem_subject":"生殖工学","subitem_subject_scheme":"Other"},{"subitem_subject":"生殖医療","subitem_subject_scheme":"Other"},{"subitem_subject":"卵母細胞","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"ブタ発育途上卵母細胞から産子を作出する培養系の構築","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ブタ発育途上卵母細胞から産子を作出する培養系の構築"}]},"item_type_id":"8","owner":"3","path":["2672"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-02"},"publish_date":"2014-10-02","publish_status":"0","recid":"13984","relation_version_is_last":true,"title":["ブタ発育途上卵母細胞から産子を作出する培養系の構築"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:05:49.470550+00:00"}