{"created":"2023-06-20T16:43:10.292818+00:00","id":13978,"links":{},"metadata":{"_buckets":{"deposit":"bd98b145-58a3-4ea4-a8b4-cba8c4298563"},"_deposit":{"created_by":3,"id":"13978","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"13978"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00013978","sets":["14:2667:2671:2672"]},"author_link":["3852"],"item_8_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Structural based fruit softening mechanism; the model of tomato fruit softening during maturation"}]},"item_8_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"科学研究費助成事業研究成果報告書 (2013. )"}]}]},"item_8_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"研究成果の概要（和文):  本研究課題は, トマト果実をモデルとして果実の発育・成熟過程における細胞壁分解酵素の一つであり, トマト果実の軟化に重要な役割を果たしていると考えられるβ-ガラクトシダーゼについて, 酵素特性や基質認識機構を明らかにすることを目的とした. トマトβ-ガラクトシダーゼ（TBG)4は果実軟化に重要な役割を果たしているため, 酵母による発現系を構築し, X線結晶構造解析を行った. その結果, 植物では初めてβ-ガラクトシダーゼの構造を明らかにすることができた. また, TBG1の酵素特性も明らかにし, TBG4とは異なる基質特異性を持つことを明らかにした.           研究成果の概要（英文):  In tomato fruit, beta-galactosidase 4 (TBG4) is an enzyme responsible for fruit softening through the degradation of beta-(1,4)-galactan in the pericarp cell wall. To gain structural in sight into the substrate specificity, we determined the crystal structures of TBG4 and its complex with beta-D-galactose. TBG4 was composed of a catalytic TIM barrel domain followed by three beta-sandwich domains. This structure is similar to other beta-galactosidase belong to GH35 families. In addition, we determined the enzymatic properties and substrate specificities of TBG1. TBG4 has substrate recognition to beta-(1,4)-linkage, however, TBG1 has beta-(1,3) and beta-(1,6)-likage substrate. In these results, TBG's act on the tomato cell wall during fruits development and  maturation, and seberal stage of tomato fruits cell walls construct/reconstruct to some components sugar as a substrate against TBG's.","subitem_description_type":"Abstract"}]},"item_8_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"研究種目： 基盤研究(C); 研究期間：2011～2013;   課題番号：23580054;   研究分野：農学;   科研費の分科・細目：農学・園芸学・造園学","subitem_description_type":"Other"}]},"item_8_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Research Paper","subitem_description_type":"Other"}]},"item_8_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_8_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学"}]},"item_8_relation_11":{"attribute_name":"著者 外部リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"http://kaken.nii.ac.jp/d/r/90326281.ja.html"}]}]},"item_8_text_10":{"attribute_name":"著者 役割","attribute_value_mlt":[{"subitem_text_value":"研究代表者"}]},"item_8_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"ISHIMARU, Megumi"}]},"item_8_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部; 准教授"}]},"item_8_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"石丸,  恵"},{"creatorName":"イシマル, メグミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3852","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90326281","nameIdentifierScheme":"研究者番号","nameIdentifierURI":" "}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-03-31"}],"displaytype":"detail","filename":"KAKEN_23580054seika.pdf","filesize":[{"value":"391.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"KAKEN_23580054seika.pdf","url":"https://kindai.repo.nii.ac.jp/record/13978/files/KAKEN_23580054seika.pdf"},"version_id":"343df683-159a-4063-a2b6-048ab38febf4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"果実軟化","subitem_subject_scheme":"Other"},{"subitem_subject":"細胞壁","subitem_subject_scheme":"Other"},{"subitem_subject":"トマト果実","subitem_subject_scheme":"Other"},{"subitem_subject":"β-ガラクトシダーゼ","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_title":"果実軟化機構の分子構造基盤研究: トマト果実をモデルとして","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"果実軟化機構の分子構造基盤研究: トマト果実をモデルとして"}]},"item_type_id":"8","owner":"3","path":["2672"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-10-02"},"publish_date":"2014-10-02","publish_status":"0","recid":"13978","relation_version_is_last":true,"title":["果実軟化機構の分子構造基盤研究: トマト果実をモデルとして"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:06:01.189970+00:00"}