@techreport{oai:kindai.repo.nii.ac.jp:00013948,
 author = {安齋,  政幸},
 month = {Jan},
 note = {研究成果の概要（和文): 卵巣内に存在する未成熟な卵子の利用は, 今後生殖補助医療分野への展開へ繋がる技術として考えられている. 申請者は未成熟卵子を用いた体外成熟操作の改善と体外受精方法を検討し, 近交系マウスを用いた産児作出に成功した. また成熟操作培地の開発を進め, 卵子初期の遺伝子発現がアミノ酸構成要素により影響を与えることを認めた. さらに小型げっ歯類を供試して体細胞の樹立を検討し、得られた体細胞ストックは、体細胞核移植により再構築卵子を作成し活性化処理後, クローン胚の作出に成功した.           研究成果の概要（英文): Use of immature oocytes present in the ovary is considered as technology leading to expansion into assisted reproductive medical field in the future. The applicant has succeeded in producing infants to consider in vitro fertilization method and improvement of in vitro maturation operation using the immature oocytes, were used inbred mice. It was also acknowledged that advances the development of mature operation medium, gene expression of initial egg affects the amino acid component. The somatic cells stock to consider the establishment of the fibroblast cells by the tested small rodents further, obtained, after activation, we have succeeded in the production of cloned embryos to create a reconstructed embryos by somatic cell nuclear transfer., 研究種目：基盤研究(C);  研究期間：2011～2013;   課題番号：23500506;   研究分野：総合領域;   科研費の分科・細目：実験動物学, application/pdf},
 title = {マウス遺伝資源の保全に対応した高度生殖工学技術の開発と体細胞核移植技術への応用},
 year = {2013},
 yomi = {アンザイ, マサユキ}
}