{"created":"2023-06-20T16:32:35.814891+00:00","id":1310,"links":{},"metadata":{"_buckets":{"deposit":"fc02cfb2-6c76-43ea-831b-053607eb0d44"},"_deposit":{"created_by":3,"id":"1310","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"1310"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00001310","sets":["14:15:788:789","21:22:662:663:790:791:792"]},"author_link":["1066","1062","1061","1059","1064","1060","1065","1063"],"item_3_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Screening of the male characteristic DNA fragment using AFLP analysis in Pacific bluefin tuna, Thunnus orientalis"}]},"item_3_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-07-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"646","bibliographicPageStart":"639","bibliographicVolumeNumber":"77","bibliographic_titles":[{"bibliographic_title":"日本水産学会誌"},{"bibliographic_title":"Nippon Suisan Gakkaishi","bibliographic_titleLang":"en"}]}]},"item_3_description_28":{"attribute_name":"識別番号 その他","attribute_value_mlt":[{"subitem_description":"10029125068","subitem_description_type":"Other"},{"subitem_description":"JST.JSTAGE/suisan/77.639","subitem_description_type":"Other"}]},"item_3_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[概要] 太平洋クロマグロ雌雄に特徴的な DNA 断片を検出する目的で AFLP 解析を行った。雌雄 4 個体ずつ計 8 個体よりゲノム DNA を調整し，64 プライマーセットで 2 次増幅した結果 1038 のバンドを検出し，内 183 が個体間で多型を示した。二次増幅を EcoRI+AGG と MseI+CAT プライマーで行った際に雄 32 個体中 29 個体，雌 32 個体中 3 個体において確認される 437 bp の断片を同定した。この断片を定量した結果，雄に於いて検出され易く，また断片の量も検出される雌より有意に（P<0.01）多かった。                [Abstract]  AFLP, amplified fragment length polymorphism screening, was conducted to identify sex characteristic DNA markers of cultured Pacific bluefin tuna (PBT), Thunnus orientalis. The AFLP screening of 4 females and 4 males with 64 primer combinations produced a total of 1038 scorable bands, of which 183 were polymorphic between individuals. Male characteristic fragments were confirmed with EcoRI+AGG and MseI+CAT primers by gel image analysis, which corresponded to 437 bp in 29 of 32 males and 3 of 32 females. Fragment detail was analyzed by electropherogram analysis and DNA quantification. The 437 bp fragment was detected in 30 individuals of average DNA concentration 0.231 ng/μL; while in females, 13 individuals were detected with average 0.080 ng/μL (P<0.01).","subitem_description_type":"Abstract"}]},"item_3_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Copyright (c) 2011 日本水産学会, rights: 本文データは学協会の許諾に基づきJ-STAGEから複製したものである, relation: isVersionOf: https://www.jstage.jst.go.jp/article/suisan/77/4/77_4_639/_article/-char/ja/","subitem_description_type":"Other"}]},"item_3_description_37":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"subitem_description":"Journal Article","subitem_description_type":"Other"}]},"item_3_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_3_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"日本水産学会"}]},"item_3_relation_24":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.2331/suisan.77.639","subitem_relation_type_select":"DOI"}}]},"item_3_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00215392","subitem_source_identifier_type":"ISSN"}]},"item_3_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"AGAWA, YASUO"},{"subitem_text_language":"en","subitem_text_value":"KOMIYA, TAKAFUMI"},{"subitem_text_language":"en","subitem_text_value":"HONRYO, TOMOKI"},{"subitem_text_language":"en","subitem_text_value":"KURATA, MICHIO"},{"subitem_text_language":"en","subitem_text_value":"OKADA, TOKIHIKO"},{"subitem_text_language":"en","subitem_text_value":"MURATA, OSAMU"},{"subitem_text_language":"en","subitem_text_value":"KUMAI, HIDEMI"},{"subitem_text_language":"en","subitem_text_value":"SAWADA, YOSHIFUMI"}]},"item_3_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学水産研究所大島実験場"},{"subitem_text_value":"近畿大学水産研究所大島実験場"},{"subitem_text_value":"近畿大学水産研究所大島実験場"},{"subitem_text_value":"近畿大学水産養殖種苗センター大島事業場"},{"subitem_text_value":"近畿大学水産養殖種苗センター大島事業場"},{"subitem_text_value":"近畿大学水産研究所白浜実験場"},{"subitem_text_value":"近畿大学水産研究所浦神実験場"},{"subitem_text_value":"近畿大学水産研究所大島実験場"}]},"item_3_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Fisheries Laboratories, Kinki University, Oshima Experiment Station"},{"subitem_text_value":"Fisheries Laboratories, Kinki University, Oshima Experiment Station"},{"subitem_text_value":"Fisheries Laboratories, Kinki University, Oshima Experiment Station"},{"subitem_text_value":"Oshima Fish Nursery Center"},{"subitem_text_value":"Oshima Fish Nursery Center"},{"subitem_text_value":"Fisheries Laboratories, Kinki University, Shirahama Experiment Station"},{"subitem_text_value":"Fisheries Laboratories, Kinki University, Uragami Experiment Station"},{"subitem_text_value":"Fisheries Laboratories, Kinki University, Oshima Experiment Station"}]},"item_3_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"阿川,  泰夫"},{"creatorName":"アガワ, ヤスオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"小宮,  貴文"},{"creatorName":"コミヤ, タカフミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"本領,  智記"},{"creatorName":"ホンリョウ, トモキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"倉田,  道雄"},{"creatorName":"クラタ, 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雄特徴的DNA断片の探索"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-21T02:35:23.025665+00:00"}