{"created":"2023-06-20T16:41:11.496363+00:00","id":11623,"links":{},"metadata":{"_buckets":{"deposit":"803c3261-e01f-49ba-b7c1-d66a9c260607"},"_deposit":{"created_by":3,"id":"11623","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"11623"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00011623","sets":["14:923:1809:3624","21:3039:3610:3625"]},"author_link":["22351","22352","22353"],"control_number":"11623","item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"23","bibliographicPageEnd":"4","bibliographicPageStart":"1","bibliographic_titles":[{"bibliographic_title":"かやのもり：近畿大学産業理工学部研究報告","bibliographic_titleLang":"ja"},{"bibliographic_title":"Reports of Faculty of Humanity-Oriented Science and Engineering, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In the present study, we investigated the intracellular delivery of siRNA using synthetic peptides as transfection reagents and the silencing effect of siRNA targeting hTERT mRNA in 3 human cancer cell lines, Jurkat, HeLa and K562. The complex of siRNA and a specific amphiphilic peptide could be effectively taken up into  cells. The complex also showed a high silencing effect against hTERT mRNA. Moreover, the combination of siRNA-NES conjugates and the amphiphilic peptides improved silencing effects up to 95.2%. The amphiphilic peptides and their hybrids showed almost no cyto-toxicity and protected siRNA against intracellular nuclease digestion in 10% FBS (half life time was over 48h).","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15100/0000011623","subitem_identifier_reg_type":"JaLC"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学産業理工学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13495801","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学産業理工学部生物環境化学科"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科"},{"subitem_text_value":"近畿大学産業理工学部生物環境化学科; 教授"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"新貝, 恭広","creatorNameLang":"ja"},{"creatorName":"シンカイ, ヤスヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"Shinkai, Yasuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"柏原, 慎一","creatorNameLang":"ja"},{"creatorName":"カシハラ, シンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Kashihara, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNames":[{"affiliationName":"近畿大学","affiliationNameLang":"ja"},{"affiliationName":"Kinki University","affiliationNameLang":"en"}]}],"creatorNames":[{"creatorName":"藤井, 政幸","creatorNameLang":"ja"},{"creatorName":"フジイ, マサユキ","creatorNameLang":"ja-Kana"},{"creatorName":"Fujii, Masayuki","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-19"}],"displaytype":"detail","filename":"AA12013209-20151210-0001.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA12013209-20151210-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/11623/files/AA12013209-20151210-0001.pdf"},"version_id":"6f57e41f-99ff-48db-88fd-3f1fd0fbe0cb"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"siRNA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"transfection","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"synthetic peptide","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"gene silencing","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング","subitem_title_language":"ja"},{"subitem_title":"〈PAPERS and REPORTS〉Non-Toxic Transfection of siRNA by Novel Synthetic Peptides and Gene Silencing","subitem_title_language":"en"}]},"item_type_id":"2","owner":"3","path":["3624","3625"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2015-12-22"},"publish_date":"2015-12-22","publish_status":"0","recid":"11623","relation_version_is_last":true,"title":["〈論文・報告〉新規合成ペプチドによるsiRNAの無毒性細胞導入と遺伝子サイレンシング"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-09-08T01:36:04.815161+00:00"}