| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2015-02-12 |
| タイトル |
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タイトル |
Inhibition of ABCG2 enhances chemo-sensitivity of murine glioma stem cell-like cells to temozolomide and reduces spheroid-forming capability |
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言語 |
en |
| 著者 |
Yoshioka, Hiromasa
Okuda, Takeshi
Fujita, Mitsugu
Inoue, Takao
Tasaki, Takayuki
Izumoto, Shuichi
Kato, Amami
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| 言語 |
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言語 |
eng |
| キーワード |
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主題 |
glioblastoma, cancer stem cell, drug resistance, ABC transporter |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
departmental bulletin paper |
| 著者 所属 |
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値 |
Department of Neurosurgery, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Neurosurgery, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Microbiology, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Pathology, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Neurosurgery, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Neurosurgery, Kinki University Faculty of Medicine |
| 著者 所属 |
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値 |
Department of Neurosurgery, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 著者所属(翻訳) |
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値 |
Kinki University |
| 版 |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 名前 |
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出版者 |
Kinki University Medical Association |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 39,
号 2,
p. 105-113,
発行日 2014-12-01
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| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
03866092 |
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
[Abstract] Current evidence indicates that glioma stem cell-like cells (GSC_S) in humans play critical roles in the pathogenesis of carcinogenesis ofglioblastoma (GBM ). The GSC_S are known to overexpress members of the adenosine triphosphate-binding cassette (ABC) family transporters to exhibit multidrug resistance. Eradication of the GSC compartment is therefore essential to achieve a stable and long-lasting remission of GBM. To elucidate the characteristics of GSC_S in detail, we generated murine GSC lines from Sleeping Beauty transposon-mediated spontaneous GBM . Using these several cell lines, we evaluated the significance of ABC transporters in the GSC kinetics by cell morphology assays, flow cytometry, and quantitativeRT-PCR for mRNA expressions. As a consequence, we show that siRNA-mediated ABCG2 inhibition enhances the sensitivity of GSC_S to temozolomide (TMZ) and in turn reduces their spheroid-forming capability. Furthermore, we show that GSC_S treated with Abcg2-specific siRNA become sensitive to TMZ and reduce their spheroid-forming capability. In conclusion, our data suggest that targeting of drug transporters in GSC_S is a promising strategy to enhance their chemo-sensitivity for achieving a longlasting remission of GBM . |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
AA0050842X-20141200-0105.pdf (5.3 MB)
