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<Originals> Establishment and characterization of mouse T-cell clones reactive to rat xeno-antigens activated via the direct or indirect recognition pathway
https://kindai.repo.nii.ac.jp/records/10567
https://kindai.repo.nii.ac.jp/records/10567ee983984-d247-44df-85c6-61b0465a23c3
| 名前 / ファイル | ライセンス | アクション |
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AA0050842-20131200-0101.pdf (637.9 kB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2014-05-27 | |||||||||
| タイトル | ||||||||||
| タイトル | <Originals> Establishment and characterization of mouse T-cell clones reactive to rat xeno-antigens activated via the direct or indirect recognition pathway | |||||||||
| 言語 | en | |||||||||
| 著者 |
Masaki, Hideyuki
× Masaki, Hideyuki
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | direct recognition, indirect recognition, T-cell clone, xeno-reactive | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 著者 所属 | ||||||||||
| 値 | Division of Diabetes, University of Massachusetts Medical School, Worcester, MA, USA, Departmen of Biochemistry, Kinki University Faculty of Medicine, | |||||||||
| 著者所属(翻訳) | ||||||||||
| 値 | Kinki University | |||||||||
| 版 | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | Kinki University Medical Association | |||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 38, 号 2, p. 101-109, 発行日 2013-12-01 |
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| ISSN | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 03866092 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | [Abstract] Xenograft rejection caused by xenoreactive T cells is a major obstacle to xenotransplantation. To analyze anti-xeno T-cell responses, xeno-reactive T-cell clones derived from C57BL/6 (B6) mice immunized with Wistar Furth (WF) rat cells were established. All CD8 clones proliferated in response to WF rat thymocytes even in the absence of B6 mouse splenocytes as antigen-presenting cells, indicating that they responded to xeno-antigen via the direct recognition pathway. In contrast, all CD4 clones proliferated against WF cells only when syngeneic splenocytes were added to the cultures, indicating that they responded via the indirect recognition pathway. All CD8 clones proliferated in response to thymocytes from WF, BB, or PVG.1R8 rats, but not to LEWIS or PVG rats. They also lysed NK-resistant C58NT(D) lymphoma cells of WF rat origin in a dosedependent manner, suggesting specificity for the rat MHC class I RT1C" allele, and their function as cytotoxic T lymphocytes. All CD4 clones proliferated against rat thymocytes of various strains only in the presence of splenocytes from B6 or BALB.B mice, which possess the H-2b haplotype, indicating that they recognize rat xeno-antigens shared between different strains in an H-2"-restricted manner. All CD4 clones secreted predominantly interferon y in response to rat xeno-antigen, suggesting that they possess Thl characteristics. Because all clones are likely to act as effector cells for xenograftrejection, they are expected to be useful for investigating mechanisms of xenograft rejection mediated by cellular immunity, and should be a good source of TcR genes for creating transgenic mice to supply naive xeno-reactive T cells. | |||||||||
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| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
