| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2011-08-22 |
| タイトル |
|
|
タイトル |
<Originals> Synergistic effect of the combination of corticosteroids and long acting-β_2 agonists on inhibiting the in vitro proliferation of T cells isolated from asthmatic patients |
|
言語 |
en |
| 著者 |
Haraguchi, Ryuta
Zushi, Hitomi
Tomita, Katsuyuki
Sano, Hiroyuki
Miyajima, Hiroyuki
Tsukamoto, Keizo
Santo, Hiroshi
Miyara, Takayuki
Higashimoto, Yuji
Toda, Yuji
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題 |
asthma, inhaled corticosteroids, synergic effect |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine, Higashiosaka City General Hospital |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 版 |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 名前 |
|
|
出版者 |
The Kinki University Medical Association |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 36,
号 1,
p. 23-27,
発行日 2011-06-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866092 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
[Abstract] The clinical efficacy of inhaled corticosteroids (ICS) combined with long-acting , β_2- agonists (LABA) has been widely demonstrated in asthma. In order to validate the benefits of the combination of these agents in vitro, we studied the inhibitory effect of fluticasone propionate (FP) or salmeterol either alone or incombination on the proliferation of T cells. In the present study, peripheral blood mononuclear cells (PBMCs) from asthmatic patients were stimulated with phytohemagglutinin (PHA) for four days in the presence of FP and/or salmeterol. We assessed cell proliferation by quantifying the fluorescence of carboxyfluorescein diacetate succinimidyl ester (CFSE) in CD3^+ cells using flow cytometry. A high concentration (10^<-8>M), but not a low concentration (10^<-12>M) of FP alone inhibited PHA-induced T cell proliferation, while salmeterol alone did not have any effect. The combination of FP (10^<-12>M) and salmeterol inhibited PHA-induced T cell proliferation, however, the combination of FP (10^<-12>M) and salbutamol, which is another shortacting β_2-agonist, did not inhibit PHA-induced T cell proliferation. This study shows that the combination of a low concentration of FP and salmeterol synergistically inhibits lymphocyte proliferation in vitro. This novel synergistic interaction of ICS and LABA may partly explain the benefits that result when these agents are given in combination to treat asthma. |
| フォーマット |
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|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
AA0050842X-20110600-0023.pdf (2.1 MB)
