| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2011-08-22 |
| タイトル |
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|
タイトル |
<Originals> Expression of the Th1 surface marker, Tim-3, on allergen-stimulated T cells of allergen-sensitve donors |
|
言語 |
en |
| 著者 |
Haraguchi, Ryuta
Zushi, Hitomi
Tomita, Katsuyuki
Yamagata, Shigeyoshi
Nishikawa, Yusaku
Sano, Akiko
Sano, Hiroyuki
Iwanaga, Takashi
Kume, Hiroaki
Toda, Yuji
|
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
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主題 |
asthma, CRTH2, Th1, Th2, Tim-3 |
| 資源タイプ |
|
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine, Higashiosaka City General Hospital |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine, Higashiosaka City General Hospital |
| 著者所属(翻訳) |
|
|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 著者所属(翻訳) |
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|
値 |
Department of Respiratory Medicine and Allergology, Kinki University Faculty of Medicine |
| 版 |
|
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 出版者 名前 |
|
|
出版者 |
The Kinki University Medical Association |
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY
巻 36,
号 1,
p. 1-6,
発行日 2011-06-01
|
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03866092 |
| 抄録 |
|
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内容記述タイプ |
Abstract |
|
内容記述 |
[Abstract] Background : The “Th2 hypothesis for asthma” remains controversial, because IFN-γ is not always suppressed in allergic asthma. Our objectives were therefore to identify the characteristics of IFN-γ-producing CD4^+ T cells that were induced by exposure to an allergen. Methods : Peripheral blood mononuclear cells (PBMC) were stimulated with house dust mites (HDM) and non-allergeic ubiquitous antigen M. tuberculosisPPD for 7 and 14 days, and then intracellular cytokines, IFN-γ and IL-4, and surface markers, Tim-3 and CRTH2, which were specifically identified in Th1 and Th2 cells, respectively, were quantified by flow cytometry. Results : We found that stimulation with HDM for 14 days generated IL-4- as well as IFN-γ-producing CD4^+ T cells (6.1±0.7％ and 24.0±6.2％, respectively). In addition, stimulation with HDM induced Tim-3 expression on IFN-γ-producing CD4^+ T cells and CRTH2 expression on IL-4-producing CD4^+ T cells (16.1±7.5％ vs. 0.9±0.5％ and 21.2±6.7％ vs. 9.6±4.7％, respectively, compared to no stimulation). On the other hand, very few IFN-γ- and IL-4-producing CD4^+ T cells stimulated with PPD expressedTim-3 and CRTH2. Conclusions : In vitro allergen-stimulated PBMCs contributed to the differentiation of Th2 as well as Th1 cells. A substantial fraction of Th1 cells expressed the expression of Tim-3, which is a death signal. |
| フォーマット |
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
AA0050842X-20110600-0001.pdf (2.7 MB)
