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Non-cleavage membrane-anchored HB-EGF enhances focal adhesion complexes in MDCK cells
https://kindai.repo.nii.ac.jp/records/10501
https://kindai.repo.nii.ac.jp/records/1050165beb90d-75cc-4c1d-90fc-d0f752fc5927
| 名前 / ファイル | ライセンス | アクション |
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AA0050842X-20081200-0035.pdf (3.1 MB)
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| Item type | ☆紀要論文 / Departmental Bulletin Paper(1) | |||||||||
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| 公開日 | 2010-07-06 | |||||||||
| タイトル | ||||||||||
| タイトル | Non-cleavage membrane-anchored HB-EGF enhances focal adhesion complexes in MDCK cells | |||||||||
| 言語 | en | |||||||||
| 著者 |
Sugimoto, Keisuke
× Sugimoto, Keisuke
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| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 主題 | Membrane-anchored HB-EGF, MDCK II, juxtacrine manner, attachment, focal adhesion, integrin | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 著者所属(翻訳) | ||||||||||
| 値 | Department of Pediatrics, Kinki University School of Medicine | |||||||||
| 版 | ||||||||||
| 出版タイプ | NA | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_be7fb7dd8ff6fe43 | |||||||||
| 出版者 名前 | ||||||||||
| 出版者 | The Kinki University Medical Association | |||||||||
| 書誌情報 |
en : ACTA MEDICA KINKI UNIVERSITY 巻 33, 号 1/2, p. 35-45, 発行日 2008-12-01 |
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| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 03866092 | |||||||||
| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Background: Heparin-binding EGF-like growth factor (HB-EGF), a member of the epithelial growth factor (EGF) family of growth factors, is first synthesized as a membrane-associated precursor (proHB-EGF). ProHB-EGF is cleaved enzymatically, resulting in the shedding of the mature secreted form (soluble HB-EGF). While soluble HB-EGF binds to and activates the EGF receptor (EGFR) as an autocrine/paracrine manner, a considerable amount of proHB-EGF remains on the cell surface, where it acts as a juxtacrine growth factor that signals to neighboring cells (juxtacrine activation). The induction of growth factors leads to the morphogenesis of focal complex processes including lamellipodia, considered to be premature cellcell contact sites. Methods: We developed Madin-Darby canine kidney (MDCK) cells stably expressing a noncleavable, membrane-anchored human HB-EGF construct (MDCK^<mem> cells) and MDCK ^<Δmem> cells in which the tyrosine phosphorylation of EGFR is prevented, to investigate the association with the expression of membrane-anchored HB-EGF and focal adhesion complex-induced morphogenesis. Results: MDCK^<mem> cells exhibited enhanced spreading and the formation of significantly extended lamellipodia compared to wild-type (WT) cells. Furthermore, there was increased tyrosine phosphorylation of Paxillin and focal adhesion kinase (FAK) in the MDCK^<mem> cells, possibly induced by the integrin-dependent activation of EGFR. MDCK^<Δmem> cells showed late attachment and no change in spreading compared to WT cells. Western blotting also showed decreased levels of phosphorylated FAK and Paxillin. Conclusion: ProHB-EGF is not only the precursor for the soluble form but also important for biological functions at focal adhesion sites. | |||||||||
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| 内容記述タイプ | Other | |||||||||
| 内容記述 | application/pdf | |||||||||
