{"created":"2023-06-20T16:40:03.730048+00:00","id":10233,"links":{},"metadata":{"_buckets":{"deposit":"6626216d-424a-4cac-a23f-707d2924cb70"},"_deposit":{"created_by":3,"id":"10233","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10233"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010233","sets":["14:923:1477:1511","21:3039:3456:3461"]},"author_link":["19296","19295"],"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"57","bibliographicPageStart":"45","bibliographicVolumeNumber":"34","bibliographic_titles":[{"bibliographic_title":"近畿大学医学雑誌"},{"bibliographic_title":"Medical journal of Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[抄録]血管内皮の酸化低比重リポ蛋白(酸化LDL)受容体として同定されたレクチン様酸化低比重リポ蛋白受容体1(LOX-1) の軟骨細胞での発現が示され，酸化LDLと軟骨変性の関連が示唆されている.本研究の目的は，酸化LDLとLOX-1の結合によりウシ培養軟骨細胞の老化が誘導されるかを検討することである. まず老化関連β-ガラクトシダーゼ染色，および， BrdU取込みによる細胞分裂能を測定した. 次に酸化LDL添加によるテロメラーゼ活性の変化， PI3 kinase/Akt経路のテロメラーゼ、活性に及ぽす影響を調べた. さらに酸化LDLによる PI3kinase/Akt経路への影響を検討した. 酸化LDL添加は培養軟骨細胞の老化関連β-ガラクトシダーゼ活性を亢進させ，細胞分裂能を低下させた. また酸化LDLは経時的，経量的にテロメラーゼ活性を抑制し，抗LOX-1抗体 (TS-20) 前処理によりテロメラーゼ活性は回復した. PI3kinase の抑制によりテロメラーゼ活性は抑制され，インスリン様成長因子-1 (IGF-l) はこの抑制を回復した.酸化LDLによりAktのリン酸化は抑制され TS-20 により回復した.すなわち酸化LDLはLOX-1との結合を介して PI3kinase/Akt経路依存性に軟骨細胞のテロメラーゼ活性を抑制する事が示された. また，これらの結果は全て継代を必要とせず24時間以内に生じた. 酸化LDLは軟骨細胞の未成熟老化を誘導するとともに，テロメラーゼ活性の抑制を介して細胞老化に関与する可能性が示された. 酸化LDLは細胞老化促進因子として軟骨変性に関与することが示唆された.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学医学部"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"03858367","subitem_source_identifier_type":"ISSN"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学医学部整形外科学教室"},{"subitem_text_value":"近畿大学医学部整形外科学教室"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"頭司,  敏史"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"赤木,  將男"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AN00063584-20090325-0045.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AN00063584-20090325-0045.pdf","url":"https://kindai.repo.nii.ac.jp/record/10233/files/AN00063584-20090325-0045.pdf"},"version_id":"cf2c6f92-82e0-47c3-a136-4e3313f60a61"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Oxidized LDL","subitem_subject_scheme":"Other"},{"subitem_subject":"LOX- 1","subitem_subject_scheme":"Other"},{"subitem_subject":"Chondrocyte","subitem_subject_scheme":"Other"},{"subitem_subject":"Premature Senescence","subitem_subject_scheme":"Other"},{"subitem_subject":"Telomerase activity","subitem_subject_scheme":"Other"},{"subitem_subject":"PI3 kinase/Akt pathway","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈原著〉酸化低比重リポ蛋白による培養ウシ関節軟骨細胞の老化誘導","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈原著〉酸化低比重リポ蛋白による培養ウシ関節軟骨細胞の老化誘導"}]},"item_type_id":"2","owner":"3","path":["1511","3461"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-01-31"},"publish_date":"2012-01-31","publish_status":"0","recid":"10233","relation_version_is_last":true,"title":["〈原著〉酸化低比重リポ蛋白による培養ウシ関節軟骨細胞の老化誘導"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:44:32.719619+00:00"}