{"created":"2023-06-20T16:39:59.174634+00:00","id":10139,"links":{},"metadata":{"_buckets":{"deposit":"709229bc-84aa-4fa5-bee3-79f4f5de51e7"},"_deposit":{"created_by":3,"id":"10139","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10139"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010139","sets":["14:923:2219:2220","21:3039:3435:3455"]},"author_link":["18965"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Molecular Dynamics Simulation with Principle Component Analysis Study on the Dynamics of DHFR Protein from Moritella profunda at High Pressure Condition"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"18","bibliographicPageStart":"9","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨] 高圧力下に生存する深海生物はその高圧力環境に適した進化を遂げている. その進化は細胞機能の殆どを司る蛋白質にも反映されていることが最近の研究から明らかとなって来た. 一般に常圧下（１気圧）に生存する蛋白質は高圧力下でその機能及び構造を変化させ多くの場合は機能が低下する. 従って高圧力下に暮らす生物（深海生物）の蛋白質は高圧力に対して最適な分子進化を遂げていることが予想される. このような背景のもと高圧力下での蛋白質構造機能を研究する実験及び理論的試みが世界中各地で進められている. 本研究では, 深海生物Moritella profunda のDHFR を, Escherichia coli のDHFR と比較することでその構造及び機能動態・構造揺らぎ, 及びその圧力依存性を様々な手法で解析した. その結果, 深海生物由来DHFR の構造揺らぎは高圧力下でも機能を失わないような独自な構造動態を示す進化を遂げていることを示唆する結果を得た.                      [Abstract] Organisms live in a variety of extreme environmental conditions such as high/low temperature, high salt concentration and also high pressure. For instance, a number of organisms have been so far discovered at deep sea. It is believed that the deep sea organisms have been evolved with adaptation to high pressure. In recent decades, proteins of the deep sea organisms have been isolated like those from thermophilic organisms. Here, in order to elucidate the adaptation mechanism to the high pressure, we have conducted extensive molecular dynamics simulation studies at high（2000 bar） and normal pressure conditions using DHFR from a deep sea organism Moritella profunda（mpDHFR）. We also simulated DHFR from Escherichia coli（ecDHFR） for omparison. We took a trajectory from the simulations and performed several analyses involving RMSD, RMSF, Gyration and principle component analysis（PCA）. We found that high pressure does not affect overall structure of DHFRs. However, large slow fluctuations that govern the functions of DHFR are significantly different between ecDHFR and mpDHFR, while the trajectory we used is rather short to sufficiently elucidate the result. The difference is likely to be caused from the E-helix fluctuation. The fluctuation differences should be responsible for the activity change of mpDHFR at high pressure.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"近畿大学先端技術総合研究所紀要編集委員会","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Yonezawa, Yasushige"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所高圧力蛋白質研究センター"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"米澤,  康滋"},{"creatorName":"ヨネザワ, ヤスシゲ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20150331-0009.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20150331-0009.pdf","url":"https://kindai.repo.nii.ac.jp/record/10139/files/AA11574630-20150331-0009.pdf"},"version_id":"f45bf58f-3a3b-4555-8041-8b5f99c9703e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"蛋白質","subitem_subject_scheme":"Other"},{"subitem_subject":"分子動力学シミュレーション","subitem_subject_scheme":"Other"},{"subitem_subject":"高圧力","subitem_subject_scheme":"Other"},{"subitem_subject":"深海生物","subitem_subject_scheme":"Other"},{"subitem_subject":"protein","subitem_subject_scheme":"Other"},{"subitem_subject":"molecular dynamics simulation","subitem_subject_scheme":"Other"},{"subitem_subject":"high pressure","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<Original Papers>高圧力下における深海生物由来ジヒドロ葉酸還元酵素DHFR 構造動態の計算科学研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<Original Papers>高圧力下における深海生物由来ジヒドロ葉酸還元酵素DHFR 構造動態の計算科学研究"}]},"item_type_id":"2","owner":"3","path":["2220","3455"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-29"},"publish_date":"2015-05-29","publish_status":"0","recid":"10139","relation_version_is_last":true,"title":["<Original Papers>高圧力下における深海生物由来ジヒドロ葉酸還元酵素DHFR 構造動態の計算科学研究"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:13.239451+00:00"}