{"created":"2023-06-20T16:39:59.131866+00:00","id":10138,"links":{},"metadata":{"_buckets":{"deposit":"6d852870-5a59-4c78-b7fb-1f476514d341"},"_deposit":{"created_by":3,"id":"10138","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10138"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010138","sets":["14:923:2219:2220","21:3039:3435:3455"]},"author_link":["18963","18962","18964","18961"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"DNA and histone methylation in early development"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"8","bibliographicPageStart":"1","bibliographicVolumeNumber":"20","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨] 哺乳類の初期胚では, 分化全能性を獲得するために, 受精直後に終末分化した精子と卵子のエピゲノムの情報がリセットされる. この現象では, 遺伝子の転写制御に関与するDNA やヒストンのメチル化及びアセチル化修飾がダイナミックに変化することが知られている. 受精後, 遺伝子の転写抑制に関わるDNAのメチル化修飾は, 酵素及びDNA 複製によって取り除かれ, その後再び, DNA メチル基転移酵素（DNAmethyltransferases, DNMTs）が働くことにより, 特定の遺伝子の転写制御が行われる. ヒストンにおいては, histone methyltransferase（HMT）によるメチル化, histone acetyltransferase（HAT）によるアセチル化などの修飾がクロマチン構造の変化を誘起させ, 転写の活性あるいは抑制に関わっている. 以上のように遺伝子の発現は, DNA やヒストン修飾の相互の働きかけによって制御されていると考えられる. したがって, 初期胚で起こるDNA 及びヒストン修飾の機構やそれらの修飾の持つ役割を知ることで, エピジェネティックリプログラミングの詳細な分子機構を深く理解することができる. さらに, それらの知見をiPS細胞作製効率の向上や分化誘導の改善に応用させることで, 再生医療や創薬分野への貢献が期待される. そこで本稿では, 哺乳類の初期胚において発生に必要な遺伝子の転写制御に関与しているDNA 及びヒストンのメチル化修飾について概説する.                      [Abstract] In mammalian early embryo, epigenome information of sperm and oocytes is reset to acquire the developmental totipotency. This phenomenon is accompanied by the dynamic changes in DNA and histone modifications. DNA methylation involved in transcriptional repression is removed by the enzymes or DNA replication after fertilization. The genome is remethylated by DNA methyltransferases（DNMTs） and the specific set of genes is transcriptionally repressed. Histone modifications such as methylation by histone methyltransferases（HMTs） and acetylation by histone acetyltransferases（HATs） also induce changes in chromatin structure and are involved in transcriptional regulation. Therefore, gene expression regulation seems to be achieved by the combination of DNA and histone modifications. Consequently, understanding mechanisms and roles of DNA and histone modifications can be a clue to understand detailed mechanism of epigenetic reprogramming. Moreover, the basic knowledge about epigenetic reprogramming to improve iPS cell conversion and induction of differentiation would contribute to the fields of regenerative medicine and innovative drug development. Here, we will review DNA and histone methylation related totranscriptional regulation of genes necessary for development in mammalian early embryo.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"近畿大学先端技術総合研究所紀要編集委員会","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Tsukaguchi, Tomomasa"},{"subitem_text_language":"en","subitem_text_value":"Morita, Kohtaro"},{"subitem_text_language":"en","subitem_text_value":"Miyamoto, Kei"},{"subitem_text_language":"en","subitem_text_value":"Matsumoto, Kazuya"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"ケンブリッジ大学"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科: 近畿大学大学院生物理工学研究科生物工学専攻"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"University of Cambridge"},{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"塚口,  智将"},{"creatorName":"ツカグチ, トモマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"守田,  昂太郎"},{"creatorName":"モリタ, コウタロウ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宮本,  圭"},{"creatorName":"ミヤモト, ケイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本,  和也"},{"creatorName":"マツモト, カズヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20150331-0001.pdf","filesize":[{"value":"760.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20150331-0001.pdf","url":"https://kindai.repo.nii.ac.jp/record/10138/files/AA11574630-20150331-0001.pdf"},"version_id":"08626553-f297-43d8-8808-1f760d181532"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"初期胚","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA メチル化","subitem_subject_scheme":"Other"},{"subitem_subject":"ヒストン修飾","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝子発現制御","subitem_subject_scheme":"Other"},{"subitem_subject":"エピジェネティックリプログラミング","subitem_subject_scheme":"Other"},{"subitem_subject":"early embryo","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA methylation","subitem_subject_scheme":"Other"},{"subitem_subject":"histone modification","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression regulation","subitem_subject_scheme":"Other"},{"subitem_subject":"epigenetic reprogramming.","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"<総説>初期胚発生におけるDNA 及びヒストンメチル化修飾の重要性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<総説>初期胚発生におけるDNA 及びヒストンメチル化修飾の重要性"}]},"item_type_id":"2","owner":"3","path":["2220","3455"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-05-29"},"publish_date":"2015-05-29","publish_status":"0","recid":"10138","relation_version_is_last":true,"title":["<総説>初期胚発生におけるDNA 及びヒストンメチル化修飾の重要性"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:15.310841+00:00"}