{"created":"2023-06-20T16:39:58.955316+00:00","id":10134,"links":{},"metadata":{"_buckets":{"deposit":"5720a0ca-98fe-4b62-91b1-fc43b5377f60"},"_deposit":{"created_by":3,"id":"10134","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10134"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010134","sets":["14:923:2219:2223","21:3039:3435:3454"]},"author_link":["18953","18952","18954","18955"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Analysis of the conservation state of 15,000 years old mammoth soft tissue by the amplification of Mc1r gene"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"61","bibliographicPageStart":"55","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨] 本実験では、約15,000 年前のマンモス皮膚、筋肉および骨髄の各組織の核DNA の保存状態を解析する事を目的に、核DNA に存在するMelanocortin type 1 receptor（Mc1r）遺伝子をPCR によって増幅し、その増幅の成否を検討した。その結果、骨髄組織由来Total DNA をPCR のTemplate とした区においてのみ、目的とする221bp のDNA 断片が増幅されたことから、マンモス骨髄組織に存在するDNA の保存状態は皮膚および筋肉組織に比べて良好であることが示された。しかし、骨髄組織由来Total DNA をTemplateとた約500bp 程度のDNA 断片を増幅するMc1r 遺伝子増幅プライマーを用いてPCR を行った結果、目的とするDNA 断片は増幅されなかった。以上の結果より、このマンモス組織においては、骨髄中の核DNAは他の皮膚または筋肉組織中の核DNA よりも保存状態が良いが、DNA の断片化が進行していることが示された。 [Abstract] In this study, to analyze the conservation state of 15,000 years old mammoth skin, muscle and bone marrow tissue by PCR, we tried to amplify the DNA fragment of Melanocortin type 1 receptor（ Mc1r）gene which exists in the cell nuclear. In results, only in the reaction in which the total DNA from the mammoth's bone marrow was used as the template of PCR, the 221bp DNA fragment was amplified. However, when we tried to amplify longer DNA fragment（ about 500bp in length） of Mc1r gene, no DNAfragment was amplified by PCR even when the total DNA from the mammoth's bone marrow was used as the template of PCR. From these results, it was suggested that the conservation state of the bone marrow is better than the skin and muscle tissue, however, the fragmentation of nuclear DNA was also heavily progressed in the bone marrow tissue.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"近畿大学先端技術総合研究所紀要編集委員会","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kondo, Kenji"},{"subitem_text_language":"en","subitem_text_value":"Yoshizaki, Takumi"},{"subitem_text_language":"en","subitem_text_value":"Kato, Hiromi"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"近藤,  健二"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"吉崎,  匡"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤,  博己"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"入谷,  明"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20140331-0055.pdf","filesize":[{"value":"565.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20140331-0055.pdf","url":"https://kindai.repo.nii.ac.jp/record/10134/files/AA11574630-20140331-0055.pdf"},"version_id":"160790f1-946a-4d86-9d5a-2bbcb1f6c8a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マンモス","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Mclr遺伝子","subitem_subject_scheme":"Other"},{"subitem_subject":"mammoth","subitem_subject_scheme":"Other"},{"subitem_subject":"PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"Mclr gene","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"(研究ノート) Mc1r 遺伝子の増幅による各種マンモス軟組織の保存状態の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"(研究ノート) Mc1r 遺伝子の増幅による各種マンモス軟組織の保存状態の検討"}]},"item_type_id":"2","owner":"3","path":["2223","3454"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-22"},"publish_date":"2014-04-22","publish_status":"0","recid":"10134","relation_version_is_last":true,"title":["(研究ノート) Mc1r 遺伝子の増幅による各種マンモス軟組織の保存状態の検討"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:22.732911+00:00"}