{"created":"2023-06-20T16:39:58.781188+00:00","id":10130,"links":{},"metadata":{"_buckets":{"deposit":"7bdf16ce-6c75-4a0d-baa6-f4f4844a849f"},"_deposit":{"created_by":3,"id":"10130","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10130"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010130","sets":["14:923:2219:2223","21:3039:3435:3454"]},"author_link":["18940","18931","18937","18939","18933","18930","18938","18934","18935","18932","18936","18941"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"A genetic resource conservation technology by establishing cultured fibroblast cells derived from two wild mice"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"23","bibliographicPageStart":"13","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨]本実験では、野生種であるアカネズミおよびカヤネズミから少量の組織サンプルを回収し効率よく安定したDNA を回収するために、バロサイクラー（Baro Cycler）を用いてDNA 抽出とその相同性について検索を行なった。さらに、両マウスの尾部から採取した組織より線維芽細胞を樹立し、染色体解析および樹立した細胞の遺伝資源保存を検討した。その結果、尾部組織および樹立線維芽細胞それぞれはシークエンス後のBLAST 検索によってデータベース上にて高い相同性を示した。また、核型解析では、それぞれの核型は高い正常性を示した。さらに、それら体細胞を用いて、電気融合法による異属間体細胞核移植を実施したところ、一部の再構築卵子は2 細胞期胚へと発生することを認めた。以上の結果から、今回樹立した野生マウス由来線維芽細胞に異常は少なく、それら細胞の遺伝子資源の保存が可能であることが示された。 [Abstract] In this study, to improve the DNA extraction efficiencies for a small amount of tissue from two wild mice, we have done a search for DNA homology with Baro Cycler. In addition, fibroblasts were also established from those mice tails. Karyotype analyses were performed on the fibroblasts, and those cells were frozen for the preservation. As a result, the frozen-thawed fibroblast cells have a high number of normal karyotype. Fibroblasts derived from organization and tail confirmed the homology sequence after BLAST search. Furthermore, these somatic cells performed somatic cell nuclear transfer（SCNT）by electrofusion method. These results of reconstructed oocytes several developed to 2-cell stage embryos. In conclusion, abnormalities in this wild mouse derived fibroblast cells were low, and thereforeconservation of genetic resources was possible in these somatic cells.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"近畿大学先端技術総合研究所紀要編集委員会","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Anzai, Masayuki"},{"subitem_text_language":"en","subitem_text_value":"Murai, Hitoshi"},{"subitem_text_language":"en","subitem_text_value":"Miyahita, Minoru"},{"subitem_text_language":"en","subitem_text_value":"Kishi, Masao"},{"subitem_text_language":"en","subitem_text_value":"Nakaya, Masataka"},{"subitem_text_language":"en","subitem_text_value":"Nishimura, Manami"},{"subitem_text_language":"en","subitem_text_value":"Sugimoto, Nao"},{"subitem_text_language":"en","subitem_text_value":"Matsuzaki, Hikaru"},{"subitem_text_language":"en","subitem_text_value":"Azuma, Rika"},{"subitem_text_language":"en","subitem_text_value":"Mitani, Tasuku"},{"subitem_text_language":"en","subitem_text_value":"Kato, Hiromi"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"富山市ファミリーパーク"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所・生石農場"},{"subitem_text_value":"近畿大学大学院生物工学専攻"},{"subitem_text_value":"近畿大学大学院生物工学専攻,九動株式会社"},{"subitem_text_value":"近畿大学生物理工学部"},{"subitem_text_value":"近畿大学生物理工学部"},{"subitem_text_value":"近畿大学生物理工学部"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所・近畿大学大学院生物工学専攻"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Kinki University"},{"subitem_text_value":"&EMPTY&"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"},{"subitem_text_value":"Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安齋,  政幸"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村井,  仁志"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"宮下,  実"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岸,  昌生"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中家,  雅隆"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西村,  愛美"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"杉本,  奈央"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松崎,  ひかる"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"東,  里香"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三谷,  匡"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤,  博己"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20140331-0013.pdf","filesize":[{"value":"867.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20140331-0013.pdf","url":"https://kindai.repo.nii.ac.jp/record/10130/files/AA11574630-20140331-0013.pdf"},"version_id":"0a917ef9-7c85-4bd9-9d68-7020acf51024"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"野生マウス","subitem_subject_scheme":"Other"},{"subitem_subject":"遺伝資源保存","subitem_subject_scheme":"Other"},{"subitem_subject":"体細胞","subitem_subject_scheme":"Other"},{"subitem_subject":"体細胞核移植","subitem_subject_scheme":"Other"},{"subitem_subject":"wild mouse","subitem_subject_scheme":"Other"},{"subitem_subject":"conservation","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic resource","subitem_subject_scheme":"Other"},{"subitem_subject":"somatic cell nuclear tranfer","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"(Original Papers) 野生マウス由来線維芽細胞の樹立による遺伝資源保存技術の一例","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"(Original Papers) 野生マウス由来線維芽細胞の樹立による遺伝資源保存技術の一例"}]},"item_type_id":"2","owner":"3","path":["2223","3454"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-04-22"},"publish_date":"2014-04-22","publish_status":"0","recid":"10130","relation_version_is_last":true,"title":["(Original Papers) 野生マウス由来線維芽細胞の樹立による遺伝資源保存技術の一例"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:29.895095+00:00"}