{"created":"2023-06-20T16:39:58.341008+00:00","id":10120,"links":{},"metadata":{"_buckets":{"deposit":"5d256288-9c8b-4e81-bdaa-436a39dc8c11"},"_deposit":{"created_by":3,"id":"10120","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10120"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010120","sets":["14:923:2219:2227","21:3039:3435:3452"]},"author_link":["18898","18900","18903","18901","18899","18902"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"〈Original Papers〉In vivo development of rabbit somatic cell nuclear transfer embryos at 14 day"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"24","bibliographicPageStart":"17","bibliographic_titles":[{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University"},{"bibliographic_title":"近畿大学先端技術総合研究所紀要","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要旨]  1997年にヒツジで初めて哺乳類の体細胞クローン作出が報告されて以来、マウスやウシ、ブタなど数多くの種で体細胞クローン産仔作出の成功が報告されているが、その産仔作出効率は未だ著しく低い。また、作出された体細胞クローンの殆どが胎盤の異常や過大仔症候群、呼吸不全などの異常を伴っている。マウス体細胞クローンにおいて、着床の前後に体細胞クローン胚の多くが致死を示すことが報告されており、また、着床後の発生においても胎盤の形成不全や胎仔の神経管・心臓・消化管・血管などに形態的異常が多く観察されている。ウサギ体細胞クローンにおいては胎仔期の発生を観察した知見は無く、体細胞クローンの体内においての発生は不明である。 本実験ではウサギ体細胞クローン胚の胚移植後の体内における着床後の発生を評価するため、胚移植後14日目のクローン胎仔および胎盤を形態的に観察した。その結果、胚移植後14 日目において着床率は7％、胎仔形成率は3％ と、非常に低率であることが分かった。また、体細胞クローン胎仔の形態的観察においては、通常の発生と違わない発生段階のものも得られたが、発生が遅延や停止しているものも得られた。胎盤においては体細胞クローンのものは重量が有意に低く、形成不全のものが観察された。     [Abstract] Since the successful cloning of sheep using somatic cell nuclear transfer（SCNT）was first reported in 1997, clone animals have been produced in many mammalian species. However, the cloning efficiency is still very low. Moreover, most of the clones have many abnormalities such as placental hyperplasia, large offspring syndrome, and obesity. In mouse SCNT cloning, it has been reported that most SCNT clones die around the time of implantation. In the development of mouse SCNT clones after implantation, many morphological abnormalities have been reported in the heart, neural tube, gut, and blood vessels. In the rabbit SCNT cloning, there have been no reports that study clone development in the fetal period. Therefore, in vivo development of rabbit SCNT clone is unknown.In this study, both cloned embryos and placentas at 14 day were morphologically-analyzed to evaluate in vivo development of rabbit SCNT embryos after implantation. The results showed both implantation rate and fetal formation rate were very low（6.9％ and 2.8％）. The clone fetuses were found to be at normal developmental stages at 14 day. However, the development of some clones had stopped or delayed. Moreover, the placenta weights of clone were significantly lower, and had morphological abnormalities.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kida, Yuta"},{"subitem_text_language":"en","subitem_text_value":"O, Noriyoshi"},{"subitem_text_language":"en","subitem_text_value":"Yamochi, Takayuki"},{"subitem_text_language":"en","subitem_text_value":"Morita, Kyohei"},{"subitem_text_language":"en","subitem_text_value":"Kishigami, Satoshi"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科:近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科:近畿大学生物理工学部　遺伝子工学科"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University:Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University:Department of Genetic Engineering, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"木田,  雄大"},{"creatorName":"キダ, ユウタ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"歐,  則克"},{"creatorName":"オウ, ノリヨシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"矢持,  隆之"},{"creatorName":"ヤモチ, タカユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"森田,  恭平"},{"creatorName":"モリタ, キョウヘイ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岸上,  哲士"},{"creatorName":"キシガミ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20120331-0017.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20120331-0017.pdf","url":"https://kindai.repo.nii.ac.jp/record/10120/files/AA11574630-20120331-0017.pdf"},"version_id":"bd9dc2c0-d2af-455a-8792-ed62690e9682"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"〈Original Papers〉胚移植後14日目におけるウサギ体細胞クローン胚の体内での発生","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"〈Original Papers〉胚移植後14日目におけるウサギ体細胞クローン胚の体内での発生"}]},"item_type_id":"2","owner":"3","path":["2227","3452"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-04-12"},"publish_date":"2012-04-12","publish_status":"0","recid":"10120","relation_version_is_last":true,"title":["〈Original Papers〉胚移植後14日目におけるウサギ体細胞クローン胚の体内での発生"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:39.922245+00:00"}