{"created":"2023-06-20T16:39:58.298638+00:00","id":10119,"links":{},"metadata":{"_buckets":{"deposit":"8353c770-adc0-45cf-a01c-a7adc5ea38e4"},"_deposit":{"created_by":3,"id":"10119","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10119"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010119","sets":["14:923:2219:2227","21:3039:3435:3452"]},"author_link":["18896","18897","18891","18889","18895","18890","18894","18892","18893"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"〈Original Papers〉Influence of L-Carnitine under in vitro maturation media in early embryo development"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"17","bibliographicPageEnd":"16","bibliographicPageStart":"9","bibliographic_titles":[{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University"},{"bibliographic_title":"近畿大学先端技術総合研究所紀要","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要約] 本研究では、卵子の細胞内運搬経路として密接な関係を示す、L- カルニチンを添加した体外成熟培地を用いて、マウス未成熟卵子への体外成熟および体外受精をおこない、その後の初期胚の発生改善に与える影響を検討した。私たちの開発した、修正TaM 培地にL- カルニチン1mM, 2mM, 5mM, 10mM を添加して体外成熟をおこなった結果、82 ～ 90％であり、非添加区（92％：905/987）との有意な差は認めず正常に発生した。次に、体外成熟を経て正常に発生した卵子の体外受精成績は、それぞれ69 ～ 80％であり、非添加区（71％：635/889）との有意な差は認めなかった。続いて、胚盤胞期胚への発生成績は、それぞれ24 ～ 46 ％ であり、2mM添加成熟培地（46 ％：136/293） および5mM添加成熟培地（37％：101/272）では、非添加区（26％：129/499）と比較し初期胚の発生に改善を認めた。さらに、初期胚の発生が改善された、2mM L- カルニチン添加区において、正常な産子への発生を認めた。これらの結果から、体外成熟培地への適切な濃度のL- カルニチン添加は、胚盤胞期胚への発生を有意に改善することが示唆された。            [Abstract] In this study, mouse immature oocytes of ICR used in vitro maturation and in vitro fertilization, since analysised early embryos development. PMSG interperitoneal administration mouse collected immature oocytes removal cumulus oocyte complexes（COCs）. In vitro maturation experimented with modified TaM medium or modified TaM medium supplement with L-Carnitine 1mM, 2mM, 5mM, and 10mM. In vitro maturation oocytes fertilized sperm of ICR. In vitro maturation medium supplement with L-Carnitine of maturation rate was 82 to 90％. There was not significant differential L-Carnitine non supplement. In vitro fertilization rate of L-Carnitine supplement was 67 to 80％.There was not significant differential L-Carnitine non supplement. In vitro early embryos development resulted 26％（129/499）, 27％（86/323）, 46％（136/293）, 37％（101/272） and 24％（71/302） respective. Moreover, Supplement withL-Carnitine 2mM and 5mM of blastcyst rate was significant higher than L-Carnitine 0mM of blastcyst rate （p＜0.05）. In conclusion, in vitro maturation medium supplement with appropriate L-Carnitine suggested that blastcyst rate was improvement.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Matsuura, Satoru"},{"subitem_text_language":"en","subitem_text_value":"Nishimura, Manami"},{"subitem_text_language":"en","subitem_text_value":"Ishizuka, Yuta"},{"subitem_text_language":"en","subitem_text_value":"Sugimoto, Nao"},{"subitem_text_language":"en","subitem_text_value":"Nakaya, Masataka"},{"subitem_text_language":"en","subitem_text_value":"Azuma, Masashi"},{"subitem_text_language":"en","subitem_text_value":"Mitani, Tasuku"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"Anzai, Masayuki"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科　生物工学専攻"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科"},{"subitem_text_value":"株式会社紀和実験動物研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学科:近畿大学大学院生物理工学研究科　生物工学専攻:近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Development of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Development of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Development of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Development of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Kiwa Laboratory Animal Co, Ltd."},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Development of Biology-Oriented Science and Technology, Kinki University:Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University:Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松浦,  悟"},{"creatorName":"マツウラ, サトル","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"西村,  愛美"},{"creatorName":"ニシムラ, マナミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石束,  祐太"},{"creatorName":"イシザカ, ユウタ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"杉本,  奈央"},{"creatorName":"スギモト, ナオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中家,  雅隆"},{"creatorName":"ナカヤ, マサタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"東,  雅志"},{"creatorName":"アズマ, マサシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三谷,  匡"},{"creatorName":"ミタニ, タスク","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安齋,  政幸"},{"creatorName":"アンザイ, マサユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20120331-0009.pdf","filesize":[{"value":"848.5 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