{"created":"2023-06-20T16:39:58.044909+00:00","id":10113,"links":{},"metadata":{"_buckets":{"deposit":"a1ec413b-c701-45c1-89e0-270591f96ecb"},"_deposit":{"created_by":3,"id":"10113","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10113"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010113","sets":["14:923:2219:2229","21:3039:3435:3451"]},"author_link":["18842","18841","18844","18843","18840","18846","18845","18848","18847"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"〈Original Papers〉Establishment of in situ hybridization technique with mouse single ovum that uses QuantiGene ViewRNA"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"16","bibliographicPageEnd":"42","bibliographicPageStart":"35","bibliographic_titles":[{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University"},{"bibliographic_title":"近畿大学先端技術総合研究所紀要","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"[要約] 私達は、Branched DNA 技術により微量mRNA の検出が可能である、QuantiGene ViewRNA を用いたin situ hybridization技術をマウス単一卵子での実験手法の確立を目的に、組織で検出できる方法を改良することにより卵細胞での評価を行なった。今回用いた卵子の成熟因子に関わるGdf9 およびハウスキーピング因子18S を検討したところ、いずれの因子において、卵子での発現を認めた。本QuantiGene ViewRNAキットを用いたin situ hybridization の手法を確立することが可能となり、今後、未成熟卵子あるいは成熟卵子そして初期胚における単一卵子での遺伝子発現の解析に有効なツールとなることが示された。  [Abstract] We evaluated ooplasm using QuantiGene ViewRNA which was able to detect a small amount of mRNA by the Branched DNA technology with a mouse single ovum. This study aimed to establish experimental method. We investigated Gdf9 which is related to oocyte maturation factor and 18S which is housekeeping factor. Hereby, it was recognized that each factor were expressed in oocytes. It was able to establish the technique of in situ hybridization using this QuantiGene ViewRNA kit. These results suggest that an effective tool for the analysis of the gene expression was mouse immature ovum or the early embryo.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Nishimura, Manami"},{"subitem_text_language":"en","subitem_text_value":"Kondo, Kenji"},{"subitem_text_language":"en","subitem_text_value":"Kiga, Keita"},{"subitem_text_language":"en","subitem_text_value":"Higashi, Kasumi"},{"subitem_text_language":"en","subitem_text_value":"Tabe, Hirokazu"},{"subitem_text_language":"en","subitem_text_value":"Nakagawa, Takao"},{"subitem_text_language":"en","subitem_text_value":"Kato, Hiromi"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"Anzai, Masayuki"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学"},{"subitem_text_value":"近畿大学生物理工学部　遺伝子工学"},{"subitem_text_value":"株式会社ベリタス"},{"subitem_text_value":"株式会社紀和実験動物研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学大学院生物理工学研究科:近畿大学生物理工学部　遺伝子工学:近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"VERITAS Co, Ltd., Tokyo"},{"subitem_text_value":"Kiwa Laboratory Animal Co, Ltd."},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Graduate School of Biology-Oriented Science and Technology, Kinki University:Department of Genetic Engineering, Kinki University:Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"西村,  愛美"},{"creatorName":"ニシムラ, マナミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"近藤,  健二"},{"creatorName":"コンドウ, ケンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"木我,  敬太"},{"creatorName":"キガ, ケイタ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"東,  佳澄"},{"creatorName":"ヒガシ, カスミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"田部,  宏和"},{"creatorName":"タベ, ヒロカズ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中川,  隆生"},{"creatorName":"ナカガワ, タカオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"加藤,  博己"},{"creatorName":"カトウ, ヒロミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"安齋,  政幸"},{"creatorName":"アンザイ, 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技術手法の確立","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"<Original Papers>QuantiGene ViewRNA を用いたマウス単一卵子でのin situ hybridization 技術手法の確立"}]},"item_type_id":"2","owner":"3","path":["2229","3451"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-05-18"},"publish_date":"2011-05-18","publish_status":"0","recid":"10113","relation_version_is_last":true,"title":["<Original Papers>QuantiGene ViewRNA を用いたマウス単一卵子でのin situ hybridization 技術手法の確立"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:46:50.462781+00:00"}