{"created":"2023-06-20T16:39:57.305884+00:00","id":10096,"links":{},"metadata":{"_buckets":{"deposit":"081febe9-f03d-45a6-b5c4-d98999a662a1"},"_deposit":{"created_by":3,"id":"10096","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10096"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010096","sets":["14:923:2219:2257","21:3039:3435:3449"]},"author_link":["18779","18778","18784","18782","18781","18777","18783","18780"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Presence of gene homologues associated with a mamalian cytochrome P450 monooxygenase (CYP1A1) and a bacterial NADH oxydase in potato genome"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1999-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"44","bibliographicPageStart":"38","bibliographic_titles":[{"bibliographic_title":"近畿大学生物理工学研究所紀要"},{"bibliographic_title":"Memoirs of the Research Institute of Biology-Oriented Science and Technology","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"異生物の既知遺伝子を使い高等植物での関連遺伝子の存在を調査する目的で、哺乳類由来の毒物代謝遺伝子Cytochrome P450 monooxygenase (CYP1A1) およびAmphibacillus xylanus由来 NADH oxidase遺伝子をプローブとしてRFLP分析を2倍体ジャガイモおよびその近縁種で行った。 ジャガイモゲノム中に CYP1A1遺伝子とバクテリアNADH oxidase遺伝子それぞれと相同性の高いDNAホモログが存在するという新たな発見があった。これは、今まで報告されていないものであり、今後植物からのこれら遺伝子の単離の先駈けとなると考えられる。　（英文） We attempted to know whether potato and its relatives could have DNA homologues associated with functional genes identified in different organisms in alternative kingdoms. Two known genes were employed for a survey using restriction fragement length polymorphism (RFLP) analysis: a mammalian cytochrome P450 1A1 which shows strong xenobiotic metabolism and a NADH oxydase gene from Amphibacillus xylanus that exhibits very rapid catalytic activities on superoxide. Genomic DNA samples from haploids from 4x potatoes (Solanum tubersoum, 2n＝2x＝24), their relatives (S.phureja, 2n＝2x=24; and S. chacoense, 2n＝2x＝24) and their 2x hybrids were digested with restriction enzymes Eco RI, EcoRV, HindIII, and Dra I then, the resulting digests were employed for Southern hybridization for RFLP. Even with a high stringency which is usually used for intraspecific genomic hybridization, Southern analysis revealed that a few copies of homologous fragements. While only a few bands were obtained per species, this is an intriguing report on the presence of the gene homologues associated with other organisms.","subitem_description_type":"Abstract"}]},"item_2_description_36":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University","subitem_description_type":"Other"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学生物理工学研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1344414X","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Watanabe, K. N"},{"subitem_text_language":"en","subitem_text_value":"Takeda, Y"},{"subitem_text_language":"en","subitem_text_value":"Tsubomoto, M"},{"subitem_text_language":"en","subitem_text_value":"Kondo, R."},{"subitem_text_language":"en","subitem_text_value":"Niimura, Y."},{"subitem_text_language":"en","subitem_text_value":"Okawa, H."},{"subitem_text_language":"en","subitem_text_value":"Hosaka, K."},{"subitem_text_language":"en","subitem_text_value":"Kasai, K."}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学生物理工学研究所 , 近畿大学生物理工学部生物工学科"},{"subitem_text_value":"近畿大学生物理工学部生物工学科"},{"subitem_text_value":"近畿大学生物理工学部生物工学科"},{"subitem_text_value":"近畿大学生物理工学部生物工学科"},{"subitem_text_value":"東京農業大学バイオサイエンス学科"},{"subitem_text_value":"神戸大学農学部"},{"subitem_text_value":"神戸大学農学部付属農場"},{"subitem_text_value":"近畿大学生物理工学部生物工学科 , 学術振興会"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邉,  和男"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹田,  靖彦"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"坪本,  真一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"近藤,  麗子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"新村,  洋一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"大川,  秀郎"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"保坂,  和良"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"笠井,  和江"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11312113-19990310-0038.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11312113-19990310-0038.pdf","url":"https://kindai.repo.nii.ac.jp/record/10096/files/AA11312113-19990310-0038.pdf"},"version_id":"5001ed21-36c5-46b3-a7a0-e50f925cc86a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ジャガイモ","subitem_subject_scheme":"Other"},{"subitem_subject":"CYP1A1","subitem_subject_scheme":"Other"},{"subitem_subject":"NADH oxydase","subitem_subject_scheme":"Other"},{"subitem_subject":"サザン分析","subitem_subject_scheme":"Other"},{"subitem_subject":"DNAホモログ","subitem_subject_scheme":"Other"},{"subitem_subject":"potato","subitem_subject_scheme":"Other"},{"subitem_subject":"Southern hybridization","subitem_subject_scheme":"Other"},{"subitem_subject":"DNA homologues","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"哺乳類由来の毒物代謝遺伝子CYP1A1およびバクテリア由来のNADHoxydase遺伝子に関連する相同性の高い遺伝子ホモログのジャガイモゲノムでの存在","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"哺乳類由来の毒物代謝遺伝子CYP1A1およびバクテリア由来のNADHoxydase遺伝子に関連する相同性の高い遺伝子ホモログのジャガイモゲノムでの存在"}]},"item_type_id":"2","owner":"3","path":["2257","3449"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-15"},"publish_date":"2010-07-15","publish_status":"0","recid":"10096","relation_version_is_last":true,"title":["哺乳類由来の毒物代謝遺伝子CYP1A1およびバクテリア由来のNADHoxydase遺伝子に関連する相同性の高い遺伝子ホモログのジャガイモゲノムでの存在"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:47:08.755386+00:00"}