@article{oai:kindai.repo.nii.ac.jp:00010088,
 author = {保坂,  卓 and 谷口,  俊仁 and 佐伯,  和弘 and 金子,  武人 and 松本,  和也 and 細井,  美彦 and 西本,  尚武 and 入谷,  明},
 issue = {3},
 journal = {近畿大学生物理工学研究所紀要, Memoirs of the Research Institute of Biology-Oriented Science and Technology},
 month = {Jan},
 note = {哺乳動物における体細胞クローン技術は、優良な遺伝形質を有する動物の増産のみならず、効率的なトランスジェニック動物作出技術への応用が進められている。今回、子ウシより採取した線維芽細胞によるクローン胚の作製および胚移植による胎子への発生を試みた。体外成熟により得られたウシ成熟卵子から核質を除去しレシピエント卵子とした。これらの卵子に数代継代したウシ線維芽細胞を挿入して作製した513個の核移植胚を電気融合したところ、63％が融合した。これら323個の再構築胚を活性化処理し、7日間培養して胚発生を観察したところ、232個 (72％)のクローン胚が卵割し、そのうち44個 (19％)が移植可能な桑実期胚および胚盤胞期胚に発生した。これら発生胚のうち10個を5頭の受卵雌に2胚ずつ移植したところ、1頭(20％)で受胎が確認された。以上より、ウシ体細胞の核移植により作製したクローン胚が胎子へ発生することが確認された。　(英文)　Production of cloned livestock from an established cultured cell line would confer various advantages in animal biotechnology, such as multiplification of valuable or endangered animals and effective production of transgenic animals. Here, we report production and in vitro development of cloned embryos with bovine fibroblasts taken from Japanese Black male calf and establishment of pregnancy with the cloned embryos following transfer into recipient animals. In vitro matured bovine oocytes were enucleated and used for recipient cytoplasm. Bovine cultured fibroblasts derived from ear skin of a Japanese Black male calf were used for nuclear donor. Couplets were produced by insertion of each somatic cell into perivitellin space of recipient oocytes. Total of 63％ out of 513 couplets was fused by electrostimulation. After activation treatment, the cloned embryos were cultured for 7 days. Frequencies of cleavage and development to the morula and blastocyst stages were 72％ and 19％, respectively. Ten morulae/blastocysts were transferred into recipient cattle nonsurgically respectively.  Ten morulae/blastocysts were transferred into recipient cattle nonsurgically ( 2 embryos/recipient). Ultrasoudsonographic diagnosis at 47 days after the transfer revealed that one (20％) pregnancy was established., 継続後誌:近畿大学先端技術総合研究所紀要 = Memoirs of Institute of Advanced Technology, Kinki University, application/pdf},
 pages = {45--51},
 title = {子ウシ線維芽細胞を用いた核移植によるクローンウシの受胎},
 year = {2000}
}