{"created":"2023-06-20T16:39:56.035189+00:00","id":10069,"links":{},"metadata":{"_buckets":{"deposit":"2d25b86a-80cb-4b25-a80d-491867b1d2eb"},"_deposit":{"created_by":3,"id":"10069","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10069"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010069","sets":["14:923:2219:2249","21:3039:3435:3446"]},"author_link":["18656","18657"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"サツマイモ遺伝資源におけるSPFMV感染診断のためのNASH法による簡易検定"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2001-06-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"14","bibliographicPageStart":"13","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"サツマイモの主要ウイルス病原であるSPFMV (sweet potato feathery mottle virus) のサツマイモ遺伝資源への感染診断を行うため、簡易検定法としてNASH (Nucleic Acid Spot Hybridization) 法の適用を行った。異なるSPFMV株の被膜タンパク質領域に関して共通した配列から設計されたPCRプライマーを用いてcDNAプローブを作成し、診断検定を行った。感度はRT-PCRに劣るものの多数の遺伝資源でのウイルス感染検定を行うための簡易性や十分な検出精度があるものと認められた。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"渡邉, 和男"},{"subitem_text_language":"en","subitem_text_value":"渡邉, 純子"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"Institute of Biology-Oriented Science and Technology"},{"subitem_text_value":"Institute of Biology-Oriented Science and Technology"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Watanabe,  Kazuo. N"},{"creatorName":"ワタナベ, カズオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe,  Junko. A"},{"creatorName":"ワタナベ, ジュンコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"18657","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20010630-0013.pdf","filesize":[{"value":"567.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20010630-0013.pdf","url":"https://kindai.repo.nii.ac.jp/record/10069/files/AA11574630-20010630-0013.pdf"},"version_id":"b076a0f0-1fe8-4fed-8fb7-c3a8caa93bcc"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Application of NASH (Nucleic Acid Spot Hybridization) in Diagnosis of SPFMV to Facilitate Indexing of Sweet-potato Germplasm","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Application of NASH (Nucleic Acid Spot Hybridization) in Diagnosis of SPFMV to Facilitate Indexing of Sweet-potato Germplasm"}]},"item_type_id":"2","owner":"3","path":["2249","3446"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-12"},"publish_date":"2010-07-12","publish_status":"0","recid":"10069","relation_version_is_last":true,"title":["Application of NASH (Nucleic Acid Spot Hybridization) in Diagnosis of SPFMV to Facilitate Indexing of Sweet-potato Germplasm"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2023-06-20T23:47:43.636645+00:00"}