| Item type |
☆紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2010-06-15 |
| タイトル |
|
|
タイトル |
ln vitro culture of CD9- and α6-integrin-expressing cells enriched by magnetic cell sorting from cryptorchid adult and pup testes in mice |
| その他（別言語等）のタイトル |
|
|
その他のタイトル |
マウス実験的停留睾丸および幼若精巣から磁気細胞分離法により濃縮したCD9 ならびにα6インテグリン発現細胞の体外培養 |
| 著者 |
Mitani, Tasuku
Ozaki, Yoshiaki
Tanaka, Yusuke
Takeuchi, Ayako
Saeki, Kazuhiro
kato, Hiromi
Matsumoto, Kazuya
Hosoi, Yoshihiko
Iritani, Akira
|
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者(英) |
|
|
言語 |
en |
|
値 |
三谷, 匡 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
尾崎, 嘉昭 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
田中, 裕介 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
竹内, 礼子 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
佐伯, 和弘 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
加藤, 博己 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
松本, 和也 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
細井, 美彦 |
| 著者(英) |
|
|
言語 |
en |
|
値 |
入谷, 明 |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Gene Control Corporation |
| 著者 所属 |
|
|
値 |
Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Gene Control Corporation |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Department of Genetic Engineering, Kinki University |
| 著者 所属 |
|
|
値 |
Institute of Advanced Technology, Kinki University , Gene Control Corporation , Department of Genetic Engineering, Kinki University |
| 版 |
|
|
出版タイプ |
NA |
|
出版タイプResource |
http://purl.org/coar/version/c_be7fb7dd8ff6fe43 |
| 出版者 名前 |
|
|
出版者 |
近畿大学先端技術総合研究所 |
| 書誌情報 |
近畿大学先端技術総合研究所紀要
en : Memoirs of Institute of Advanced Technology, Kinki University
号 11,
p. 23-34,
発行日 2006-03-01
|
| ISSN |
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|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
13468693 |
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Recently, studies on cell surface markers of spermatogonia in combination with germ cell transplantation technique have made possible their functional analysis and the germline stem cells（GS cell）have established. GS cells are downstream of the stem cells such as ES cells and embryonic germ cells, namely EG cells, which are derived from PGCs. Therefore, GS cells are expected to be useful for the production of genetically-modified animals. In this study, we examined enrichment of GS cells expressing CD9 or α6-integrin from C57BL/6J cryptorchid adult and ICR pup（6-8 dpp）testes by magnetic cell sorting（MACS）and cultivation of those cells in vitro. Flow cytometric analyses demonstrated that MACS effectively enriched CD9-positive（CD9^+）and α6-integrin-positive （α6-integrin^+）cells most effectively from pup testis. Therefore, CD9^+ and α6-integrin^+ cells from pup testes were used for the following cultivation. Cells proliferated in the first few days in suspension, subsequently attached to the culture plates and formed colonies after 3-5 days of culture. Those GS-like cells were passaged on the feeder layers and showed continuous proliferation for more than 2 months. Immunocytochemical and FCM analyses demonstrated that those cells maintained the expression of CD9, α6-integrin and Oct4. These findings indicate that the CD9^+ and α6-integrin^+ cells collected from mouse pup testes have GS cell properties. Now, transplantation of GS-like cells into testis of recipient W mice which lack spermatogenesis is under way to evaluate their ability of spermatogenesis. |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
AA11574630-20060331-0023.pdf (3.8 MB)
