{"created":"2023-06-20T16:39:53.239873+00:00","id":10021,"links":{},"metadata":{"_buckets":{"deposit":"71d1471b-7a91-44f0-8e5e-dfab5ee8554e"},"_deposit":{"created_by":3,"id":"10021","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10021"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010021","sets":["14:923:2219:2237","21:3039:3435:3439"]},"author_link":["18467","18468","18464","18457","18459","18461","18462","18466","18463","18465","18458","18460"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"DNA methylation levels in cloned bovine embryos with early G1 cells"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"12","bibliographicPageEnd":"31","bibliographicPageStart":"25","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although cloning by somatic cell nuclear transfer （NT） has achieved in various mammalian species, its efficiency has been extremely low. Recently, enhanced development to full ‒ term was obtained with NT embryos with bovine early G1 cells rather than NT embryos with serum starved （G0） cells. In this study, we examined genome ‒ wide DNA methylation of cloned bovine embryos with early G1 cells by immunostaining technique with an antibody to 5‒methyl cytosine. Somatic cell nuclear transfer embryos were produced by bovine enucleated matured oocytes electrofusing with bovine G0‒ or eG1 ‒ phase fibroblasts. Levels of DNA methylation of the NT embryos both with G0 of eG1 cells were compared to those of in ‒ vitro fertilized embryos at 8‒ cell stage. Our results indicated that regardless of the donor cell type, greater relative levels of DNA methylation were observed in NT embryos than those of IVF embryos. However, the relative levels of DNA methylation of eG1‒NT embryos were lower than those of G0‒NT embryos. Thus, eG1‒NT embryos were genome ‒ widely hypomethylated compared with G0‒NT embryos. These results suggest that a possible reason of the high success rate of eG1‒NT embryos may be due to their lower methylation levels than those of G0‒NT embryos at 8‒cell stage.","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Iwamoto, Daisaku"},{"subitem_text_language":"en","subitem_text_value":"Nakamoto, Yoshiyuki"},{"subitem_text_language":"en","subitem_text_value":"Kasamatsu, Aya"},{"subitem_text_language":"en","subitem_text_value":"Tatemizo, Atsuhiro"},{"subitem_text_language":"en","subitem_text_value":"Taniguchi, Shunji"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"Matsumoto, Kazuya"},{"subitem_text_language":"en","subitem_text_value":"Ideta, Atsushi"},{"subitem_text_language":"en","subitem_text_value":"Urakawa, Manami"},{"subitem_text_language":"en","subitem_text_value":"Aoyagi, Yoshito"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"},{"subitem_text_language":"en","subitem_text_value":"Saeki, Kazuhiro"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科 , わかやま産業振興財団"},{"subitem_text_value":"近畿大学大学院生物理工学研究科 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学大学院生物理工学研究科 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学先端技術総合研究所"},{"subitem_text_value":"全農ETセンター"},{"subitem_text_value":"全農ETセンター"},{"subitem_text_value":"全農ETセンター"},{"subitem_text_value":"近畿大学大学院生物理工学研究科 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学大学院生物理工学研究科 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学先端技術総合研究所"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Department of Genetic Engineering, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University , Wakayama Industry Promotion Foundation"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University , Department of Genetic Engineering, Kinki University , Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University , Department of Genetic Engineering, Kinki University , Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"ZEN ‒ NOH Embryo Transfer Center"},{"subitem_text_value":"ZEN ‒ NOH Embryo Transfer Center"},{"subitem_text_value":"ZEN ‒ NOH Embryo Transfer Center"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University , Department of Genetic Engineering, Kinki University , Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology ‒ Oriented Science and Technology, Kinki University , Department of Genetic Engineering, Kinki University , Institute of Advanced Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"岩本,  太作"},{"creatorName":"イワモト, ダイスケ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中本,  善之"},{"creatorName":"ナカモト, ヨシユキ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"笠松,  礼"},{"creatorName":"カサマツ, アヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"立溝,  篤宏"},{"creatorName":"タテミゾ, アツヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"谷口,  俊仁"},{"creatorName":"タニグチ, シュンジ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本,  和也"},{"creatorName":"マツモト, カズヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"出田,  篤司"},{"creatorName":"イデタ, アツシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"浦川,  真実"},{"creatorName":"ウラカワ, マナミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"青柳,  敬人"},{"creatorName":"アオヤギ, ヨシト","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"入谷,  明"},{"creatorName":"イリタニ, アキラ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐伯,  和弘"},{"creatorName":"サエキ, カズヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2016-02-18"}],"displaytype":"detail","filename":"AA11574630-20070331-0025.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AA11574630-20070331-0025.pdf","url":"https://kindai.repo.nii.ac.jp/record/10021/files/AA11574630-20070331-0025.pdf"},"version_id":"394df7a7-77c1-4132-b497-39cac2113558"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin 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