{"created":"2023-06-20T16:39:52.915799+00:00","id":10016,"links":{},"metadata":{"_buckets":{"deposit":"37886991-7aeb-4c6e-95aa-55a6c7d5095c"},"_deposit":{"created_by":3,"id":"10016","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"10016"},"status":"published"},"_oai":{"id":"oai:kindai.repo.nii.ac.jp:00010016","sets":["14:923:2219:2235","21:3039:3435:3438"]},"author_link":["18424","18426","18428","18425","18430","18427","18431","18432","18429","18433"],"item_2_alternative_title_3":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Gene expression activity of the LTR of murine IAP-like bovine retrotransposon"}]},"item_2_biblio_info_21":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"13","bibliographicPageEnd":"70","bibliographicPageStart":"61","bibliographic_titles":[{"bibliographic_title":"近畿大学先端技術総合研究所紀要"},{"bibliographic_title":"Memoirs of Institute of Advanced Technology, Kinki University","bibliographic_titleLang":"en"}]}]},"item_2_description_33":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"げっ歯類のゲノム中には、Intracisternal A Particle（IAP）と呼ばれるレトロトランスポゾンが存在する。IAP は、その両端にLong Terminal Repeat（LTR）と呼ばれる、強力な転写プロモーター活性やエンハンサー活性を持つ、長い末端反復配列を持つ（Dewannieux et al., 2004）。このLTR 配列の転写プロモーター活性やエンハンサー活性は、ゲノム中のIAP 挿入部位の前後に存在する遺伝子の発現に影響を及ぼすことが報告されている（Barbot et al., 2002, Jaenisch et al.,2003, Morgan et al., 1999）。実験動物であるマウスにおいては、IAP を含むレトロトランスポゾンについて多くの研究が報告されているが、家畜であるウシにおいては、レトロトランスポゾンに関する研究はほとんどなされていない。ウシゲノムプロジェクトが進行中である現在、ウシゲノムにおけるトランスポゾンなどの機能未知であるnon-coding 領域の研究を行うことは、ウシゲノムの機能解析においても有効であると考えられる。そこで本研究では、当研究室において得られたマウスIAP 様ウシレトロトランスポゾンのLTR 配列を転写プロモーターとして持つ、Enhanced Green Fluorescent Protein（EGFP）発現ベクターを構築し、マウス線維芽細胞、ウシ線維芽細胞およびマウスES 細胞へ導入し、ウシゲノム中に存在するマウスIAP 様ウシレトロトランスポゾンのLTR 配列の転写プロモーター活性について検討した。","subitem_description_type":"Abstract"}]},"item_2_description_41":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_2_publisher_14":{"attribute_name":"出版者 名前","attribute_value_mlt":[{"subitem_publisher":"近畿大学先端技術総合研究所"}]},"item_2_source_id_22":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"13468693","subitem_source_identifier_type":"ISSN"}]},"item_2_text_7":{"attribute_name":"著者(英)","attribute_value_mlt":[{"subitem_text_language":"en","subitem_text_value":"Kato, Hiromi"},{"subitem_text_language":"en","subitem_text_value":"Kawano, Yoshihiro"},{"subitem_text_language":"en","subitem_text_value":"Takahashi, Yoshie"},{"subitem_text_language":"en","subitem_text_value":"Koda, Toshio"},{"subitem_text_language":"en","subitem_text_value":"Kishimoto, Manabu"},{"subitem_text_language":"en","subitem_text_value":"Mitani, Tasuku"},{"subitem_text_language":"en","subitem_text_value":"Matsumoto, Kazuya"},{"subitem_text_language":"en","subitem_text_value":"Saeki, Kazuhiro"},{"subitem_text_language":"en","subitem_text_value":"Hosoi, Yoshihiko"},{"subitem_text_language":"en","subitem_text_value":"Iritani, Akira"}]},"item_2_text_8":{"attribute_name":"著者 所属","attribute_value_mlt":[{"subitem_text_value":"近畿大学先端技術総合研究所 , 財団法人わかやま産業振興財団"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学生物理工学部遺伝子工学科"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学先端技術総合研究所"},{"subitem_text_value":"近畿大学先端技術総合研究所 , 財団法人わかやま産業振興財団 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学先端技術総合研究所 , 財団法人わかやま産業振興財団 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学先端技術総合研究所 , 財団法人わかやま産業振興財団 , 近畿大学生物理工学部遺伝子工学科 , 近畿大学大学院生物理工学研究科生物工学専攻"},{"subitem_text_value":"近畿大学先端技術総合研究所 , 財団法人わかやま産業振興財団 , 近畿大学大学院生物理工学研究科生物工学専攻"}]},"item_2_text_9":{"attribute_name":"著者所属(翻訳)","attribute_value_mlt":[{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University , School of Biology-Oriented Science and Technology, Kinki University , Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University , School of Biology-Oriented Science and Technology, Kinki University , Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University , School of Biology-Oriented Science and Technology, Kinki University , Division of Biological Science, Graduate School of Biology-Oriented Science and Technology, Kinki University"},{"subitem_text_value":"Institute of Advanced Technology, Kinki University , School of Biology-Oriented Science and Technology, Kinki University"}]},"item_2_version_type_12":{"attribute_name":"版","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43","subitem_version_type":"NA"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤,  博己"},{"creatorName":"カトウ, ヒロミ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"河野,  善衛"},{"creatorName":"カワノ, ヨシヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"高橋,  佳江"},{"creatorName":"タカハシ, ヨシエ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"甲田,  俊夫"},{"creatorName":"コウダ, トシオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岸本,  学"},{"creatorName":"キシモト, マナブ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"三谷,  匡"},{"creatorName":"ミタニ, タスク","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本,  和也"},{"creatorName":"マツモト, カズヤ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"佐伯,  和弘"},{"creatorName":"サエキ, カズヒロ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"細井,  美彦"},{"creatorName":"ホソイ, ヨシヒコ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"入谷,  明"},{"creatorName":"イリタニ, 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